Although several studies have analyzed the fatty acid profile of phospholipids (PL) and, to a lesser degree, triacylglycerols (TG) in one or more tissues concurrently, a systematic comparison of the fatty acid composition of different tissues and/or lipid classes is lacking. The purpose of the present study was to compare the fatty acid composition of major lipid classes (PL and TG) in the rat serum, soleus muscle, extensor digitorum longus muscle and the heart. Lipids were extracted from these tissues and analyzed by a combination of thin-layer chromatography and gas chromatography. We found many significant differences in various tissues and lipid classes. Serum had the most distinct fatty acid profile in PL but this "uniqueness" was less apparent in TG, where differences among tissues were in general less frequent than in PL. These two skeletal muscles exhibited similar fatty acid composition in both lipid classes despite their different muscle fiber type composition, denoting that fiber type is not a major determinant of the fatty acid composition of rat skeletal muscle. The fatty acid profile of heart PL was the most different from that of the other tissues examined. PL were rich in polyunsaturated fatty acids, whereas TG were rich in monounsaturated fatty acids. Although the reasons for the differences in fatty acid profile among the tissues examined are largely unknown, it is likely that these differences have an impact upon numerous biological functions.
- MeSH
- acetyltransferasy metabolismus MeSH
- desaturasy mastných kyselin metabolismus MeSH
- elongasy mastných kyselin MeSH
- fosfolipidy chemie MeSH
- kosterní svaly chemie enzymologie MeSH
- krysa rodu Rattus MeSH
- mastné kyseliny analýza krev MeSH
- myokard chemie enzymologie MeSH
- nenasycené mastné kyseliny analýza krev MeSH
- potkani Wistar MeSH
- triglyceridy chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- acetyltransferasy MeSH
- desaturasy mastných kyselin MeSH
- elongasy mastných kyselin MeSH
- fosfolipidy MeSH
- mastné kyseliny MeSH
- nenasycené mastné kyseliny MeSH
- triglyceridy MeSH
Brown adipose tissue (BAT) physiology and imaging have recently attracted considerable attention. BAT is characterized both by enhanced perfusion and increased mitochondrial activity. (99m)Tc-sestamibi is a lipophilic cationic tracer that concentrates in mitochondria. Data on the accumulation of (99m)Tc-sestamibi in BAT are currently lacking. This study investigates the in vivo (99m)Tc-sestamibi uptake in rat BAT. (99m)Tc-sestamibi was administered in male Wistar rats of various age and body size. (99m)Tc-sestamibi uptake was measured in vitro in BAT and white fat (WF) together with cytochrome c oxidase activity. Both (99m)Tc-sestamibi uptake and cytochrome c oxidase activity were higher in BAT than in WF (P<0.05). (99m)Tc-Sestamibi uptake in both BAT and WF was negatively related to body weight (r = -0.96 and -0.89, respectively) as was the BAT/WF uptake ratio (r = -0.85). These data show a higher (99m)Tc-sestamibi uptake in BAT compared to WF, in agreement with the high mitochondrial content and respiratory activity of the former. The strong negative correlation between (99m)Tc-sestamibi uptake in BAT and body weight (negative allometry), is in accordance to increased needs of thermogenesis in smaller animals. Implications of increased (99m)Tc-sestamibi uptake in BAT in radionuclide imaging are also discussed.
- MeSH
- bílá tuková tkáň metabolismus MeSH
- hnědá tuková tkáň diagnostické zobrazování metabolismus fyziologie MeSH
- krysa rodu Rattus MeSH
- mitochondrie diagnostické zobrazování metabolismus fyziologie MeSH
- potkani Wistar MeSH
- radiofarmaka metabolismus MeSH
- radioisotopová scintigrafie MeSH
- respirační komplex IV metabolismus MeSH
- stárnutí metabolismus MeSH
- technecium 99mTc sestamibi metabolismus MeSH
- tělesná hmotnost MeSH
- termogeneze * MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- radiofarmaka MeSH
- respirační komplex IV MeSH
- technecium 99mTc sestamibi MeSH
The study investigates the effect of administered estrogen on plasma creatine kinase (CK) and lactate dehydrogenase (LD) levels in female ovariectomized rats after downhill running. Rats ovariectomized before sexual maturity were subcutaneously implanted with pellets containing 17 beta-estradiol or placebo. Three weeks later they were subjected to a 90-min intermittent downhill running protocol. Blood samples were obtained from the jugular vein immediately after and 72 h after exercise for determination of plasma CK, LD and 17 beta-estradiol levels. A two-way analysis of variance was used for data evaluation. Plasma CK and LD levels were significantly lower (p<0.05) in the estrogen-supplemented, ovariectomized animals which suggests that less muscle damage occurred compared to the controls immediately and 72 h after exercise. Estrogens may have a protective effect on muscle tissue possibly due to their antioxidant and membrane stabilizing properties.
- MeSH
- běh fyziologie MeSH
- časové faktory MeSH
- estradiol farmakologie MeSH
- estrogeny farmakologie MeSH
- kreatinkinasa krev MeSH
- krysa rodu Rattus MeSH
- L-laktátdehydrogenasa krev MeSH
- ovarektomie MeSH
- potkani Wistar MeSH
- tělesná hmotnost účinky léků fyziologie MeSH
- zátěžový test MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- estradiol MeSH
- estrogeny MeSH
- kreatinkinasa MeSH
- L-laktátdehydrogenasa MeSH
