The field of biosensing would significantly benefit from a disruptive technology enabling flexible manufacturing of uniform electrodes. Inkjet printing holds promise for this, although realizing full electrode manufacturing with this technology remains challenging. We introduce a nitrogen-doped carboxylated graphene ink (NGA-ink) compatible with commercially available printing technologies. The water-based and additive-free NGA-ink was utilized to produce fully inkjet-printed electrodes (IPEs), which demonstrated successful electrochemical detection of the important neurotransmitter dopamine. The cost-effectiveness of NGA-ink combined with a total cost per electrode of $0.10 renders it a practical solution for customized electrode manufacturing. Furthermore, the high carboxyl group content of NGA-ink (13 wt%) presents opportunities for biomolecule immobilization, paving the way for the development of advanced state-of-the-art biosensors. This study highlights the potential of NGA inkjet-printed electrodes in revolutionizing sensor technology, offering an affordable, scalable alternative to conventional electrochemical systems.
- Klíčová slova
- Biosensor, Dopamine detection, Functionalized graphene ink, Inkjet printing,
- MeSH
- biosenzitivní techniky * přístrojové vybavení MeSH
- design vybavení MeSH
- dopamin * analýza MeSH
- dusík chemie MeSH
- elektrochemické techniky * metody přístrojové vybavení MeSH
- elektrody MeSH
- grafit * chemie MeSH
- inkoust * MeSH
- lidé MeSH
- tiskařství * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dopamin * MeSH
- dusík MeSH
- grafit * MeSH
Tackling the current problem of antimicrobial resistance (AMR) requires fast, inexpensive, and effective methods for controlling and detecting antibiotics in diverse samples at the point of interest. Cost-effective, disposable, point-of-care electrochemical biosensors are a particularly attractive option. However, there is a need for conductive and versatile carbon-based materials and inks that enable effective bioconjugation under mild conditions for the development of robust, sensitive, and selective devices. This work describes a simple and fast methodology to construct an aptasensor based on a novel graphene derivative equipped with alkyne groups prepared via fluorographene chemistry. Using click chemistry, an aptamer is immobilized and used as a successful platform for the selective determination of ampicillin in real samples in the presence of interfering molecules. The electrochemical aptasensor displayed a detection limit of 1.36 nM, high selectivity among other antibiotics, the storage stability of 4 weeks, and is effective in real samples. Additionally, structural and docking simulations of the aptamer shed light on the ampicillin binding mechanism. The versatility of this platform opens up wide possibilities for constructing a new class of aptasensor based on disposable screen-printed carbon electrodes usable in point-of-care devices.
- Klíčová slova
- antibiotic detection, aptamers, biosensors, graphene acid, screen printed carbon electrodes,
- MeSH
- alkyny MeSH
- ampicilin MeSH
- antibakteriální látky MeSH
- aptamery nukleotidové * chemie MeSH
- biosenzitivní techniky * metody MeSH
- click chemie MeSH
- elektrochemické techniky metody MeSH
- elektrody MeSH
- grafit * chemie MeSH
- limita detekce MeSH
- uhlík chemie MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkyny MeSH
- ampicilin MeSH
- antibakteriální látky MeSH
- aptamery nukleotidové * MeSH
- grafit * MeSH
- uhlík MeSH
- zlato MeSH
