Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development-specific requirements of B1-type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1-type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1-type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA-TUBULIN COMPLEX PROTEIN 3-INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1-type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s.
- Klíčová slova
- CDK, CYCB1, endosperm, microtubule nucleation, mitosis,
- MeSH
- Arabidopsis * genetika metabolismus MeSH
- buněčné dělení * MeSH
- cyklin B1 * genetika metabolismus MeSH
- mikrotubuly * metabolismus MeSH
- mitóza genetika MeSH
- proteiny huseníčku * genetika metabolismus MeSH
- transportní proteiny MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyklin B1 * MeSH
- GIP1 protein, Arabidopsis MeSH Prohlížeč
- proteiny huseníčku * MeSH
- transportní proteiny MeSH
