Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five Parabacteroides distasonis strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of P. distasonis DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4+ T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD.
- Klíčová slova
- IBD, colitis, functional screening, holobiont, immune response, live biotherapeutic products (LBP), microbiota, probiotics,
- MeSH
- Bacteroidetes genetika imunologie izolace a purifikace MeSH
- Caco-2 buňky MeSH
- DNA bakterií genetika metabolismus MeSH
- dospělí MeSH
- feces mikrobiologie MeSH
- idiopatické střevní záněty imunologie mikrobiologie MeSH
- kolitida chemicky indukované imunologie mikrobiologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- kyselina trinitrobenzensulfonová škodlivé účinky MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- novorozenec MeSH
- regulační T-lymfocyty imunologie MeSH
- střevní mikroflóra imunologie MeSH
- střevní sliznice imunologie MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- myši MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA bakterií MeSH
- kyselina trinitrobenzensulfonová MeSH
