Beside the key inhibition of acetylcholinesterase (AChE), involvement of oxidative stress in organophosphate (OP)-induced toxicity has been supported by experimental and human studies. On the other hand, according to our best knowledge, possible antioxidant properties of oximes, the only causal antidotes to OP-inhibited AChE, have been examined only by a few studies. Thus, we have determined the effect of four conventional (obidoxime, trimedoxime, pralidoxime, asoxime) and two promising experimental oximes (K027, K203) on dichlorvos (DDVP)-induced oxidative changes in vivo. Wistar rats (5/group) were treated with oxime (5% LD50 i.m) immediately after DDVP challenge (75% LD50 s.c). Oxidative stress biomarkers were determined in plasma and brain 60 min after the treatment: prooxidative-superoxide anion (O2·-) and total oxidative status (TOS); antioxidative-superoxide dismutase (SOD), total thiol (SH) groups, total antioxidant status (TAS) and paraoxonase (PON1); tissue oxidative stress burden-prooxidative-antioxidative balance (PAB) and oxidative stress index (OSI); oxidative tissue damage-malondialdehyde (MDA) and advanced oxidation protein products (AOPP). All oximes were able to attenuate DDVP-induced oxidative stress in rat plasma and brain. Changes of determined parameters in brain were not as prominent as it was seen in plasma. Based on OSI, better abilities of oxime K027, K203 and obidoxime to maintain DDVP-induced oxidative stress in rat brain were shown as compared to trimedoxime, pralidoxime and asoxime. Oximes can influence the complex in vivo redox processes that might contribute to their overall therapeutic efficacy. Further research is needed to understand the underlying molecular mechanisms involved in this phenomenon.

• Klíčová slova
• Antioxidats, Dichlorvos, Efficacy, Oxime K027, Oxime K203, Pro-oxidants,
• MeSH
• aryldialkylfosfatasa krev   MeSH
• biologické markery krev   MeSH
• cholinesterasové inhibitory farmakologie   MeSH
• dichlorvos toxicita   MeSH
• krysa rodu Rattus MeSH
• malondialdehyd krev   MeSH
• mozek účinky léků   MeSH
• obidoxim chlorid farmakologie   MeSH
• otrava organofosfáty farmakoterapie   MeSH
• oxidační stres účinky léků   MeSH
• oximy farmakologie   MeSH
• pralidoximové sloučeniny MeSH
• pyridinové sloučeniny farmakologie   MeSH
• superoxiddismutasa krev   MeSH
• trimedoxim farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aryldialkylfosfatasa MeSH
• asoxime chloride MeSH Prohlížeč
• biologické markery MeSH
• cholinesterasové inhibitory MeSH
• dichlorvos MeSH
• malondialdehyd MeSH
• obidoxim chlorid MeSH
• oximy MeSH
• Pon1 protein, rat MeSH Prohlížeč
• pralidoxime MeSH Prohlížeč
• pralidoximové sloučeniny MeSH
• pyridinové sloučeniny MeSH
• superoxiddismutasa MeSH
• trimedoxim MeSH