The distribution of HLA class II DRB1 and DQB1 alleles in cervical carcinoma patients was compared with the frequency of these alleles found in healthy population living in the Czech Republic. The RFLP analysis and PCR-SSP were used for DNA typing. Although the differences in the frequency of DRB1*03, DQB1*02 and DQB1*0303 alleles between the cases and the controls were rather large, corrected P values did not reach significance.
- MeSH
- alely MeSH
- dospělí MeSH
- dysplazie děložního hrdla epidemiologie genetika imunologie MeSH
- frekvence genu * MeSH
- genetická predispozice k nemoci MeSH
- genotyp MeSH
- geny MHC třídy II * MeSH
- HLA-DQ antigeny genetika MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DR antigeny genetika MeSH
- HLA-DRB1 řetězec MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory děložního čípku epidemiologie genetika imunologie MeSH
- polymerázová řetězová reakce MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- polymorfismus konformace jednovláknové DNA MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- spinocelulární karcinom epidemiologie genetika imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
- Názvy látek
- HLA-DQ antigeny MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DQB1 antigen MeSH Prohlížeč
- HLA-DR antigeny MeSH
- HLA-DRB1 řetězec MeSH
The frequencies of phenotypes, alleles and allelic subtypes of DRB1 and DQB1 HLA loci in 420 unrelated individuals from the Czech population were determined. The frequencies of DPB1 alleles of the HLA locus were determined in 92 individuals. The assays were performed using the polymerase chain reaction (PCR) method or the restriction fragment length polymorphism (RFLP) analysis. The most frequent DRB1 allele was *07, the most frequent DQB1 allele was *03 and the most frequent DPB1 allele detected was *04. These assays define the extent of polymorphism of the HLA system and are useful for determining the selection strategy of HLA-identical donor-recipient pair suitable for bone marrow transplantation.
- MeSH
- alely MeSH
- frekvence genu * MeSH
- genotyp MeSH
- geny MHC třídy II * MeSH
- HLA-DP antigeny genetika MeSH
- HLA-DP beta řetězec MeSH
- HLA-DQ antigeny genetika MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DR antigeny genetika MeSH
- HLA-DRB1 řetězec MeSH
- lidé MeSH
- transplantace kostní dřeně MeSH
- získávání tkání a orgánů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- HLA-DP antigeny MeSH
- HLA-DP beta řetězec MeSH
- HLA-DPB1 antigen MeSH Prohlížeč
- HLA-DQ antigeny MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DQB1 antigen MeSH Prohlížeč
- HLA-DR antigeny MeSH
- HLA-DRB1 řetězec MeSH
The use of HLA-DRB1 and -DQB1 polymerase chain reaction-sequence-specific primer (PCR-SSP) typing at different levels of resolution for MLR prediction was assessed in 54 HLA-A and -B matched donor/recipient unrelated pairs and 89 HLA-A and -B identical siblings. Graft-versus-host (GvH) direction one-way MLR was evaluated unless stated otherwise. The typing of DRB1 alleles satisfactory for MLR prediction in HLA identical siblings (P = 0.0015) does not appear to be sufficient in matched unrelated pairs (P = 0.2407). Using more discriminatory PCR-SSP typing, the disparity in DRB1 allelic subtypes was predominantly found in the category of DRB1 allele compatible, MLR positive unrelated pairs. Besides, DRB1 allelic subtype mismatches were revealed in five of the forty-one DRB1 allele compatible, MLR negative unrelated pairs. More discriminatory typing made the correlation between DRB1 compatibility and MLR negativity extremely significant (P = 0.0001). As for these five exceptional cases, the reciprocal host-versus-graft (HvG) direction MLR was considered, too. This allowed HLA-D disparity to be disclosed in two of them. An uninterpretable result reflecting defective MLR reactivity occurred in one case. Negative reciprocal MLR in the last two DRB1 allelic subtype incompatible pairs is hardly to explain without postulation of MLR silent DRB1 allelic subtype mismatches. An analysis in unrelated pairs showed a role of some DQB1 gene products in the proliferative response too. GvH direction positive MLR was found in two HLA identical siblings among the 89 tested. The DPB1 incompatibility detected in one of them could be a potential cause of proliferative response but MLR reactivity in the other, DPB1 identical, pair cannot be interpreted easily.
- MeSH
- alely MeSH
- dárci tkání MeSH
- HLA-A antigeny genetika MeSH
- HLA-B antigeny genetika MeSH
- HLA-D antigeny genetika MeSH
- HLA-DQ antigeny genetika MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DR antigeny genetika MeSH
- HLA-DRB1 řetězec MeSH
- hodnotící studie jako téma MeSH
- lidé MeSH
- nukleární rodina MeSH
- polymerázová řetězová reakce metody MeSH
- prediktivní hodnota testů MeSH
- test smíšené lymfocytární kultury * MeSH
- testování histokompatibility metody MeSH
- transplantace kostní dřeně imunologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- HLA-A antigeny MeSH
- HLA-B antigeny MeSH
- HLA-D antigeny MeSH
- HLA-DQ antigeny MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DQB1 antigen MeSH Prohlížeč
- HLA-DR antigeny MeSH
- HLA-DRB1 řetězec MeSH
The search for compatible donors is based on the HLA types of donors and recipients. HLA-A, -B and -DRB1 antigens or alleles must be unequivocally typed in donors and recipients. The typing of HLA-DQB, -DPB and -C gene products is also useful to characterize the HLA phenotype, but it is not absolutely necessary in the donor selection. The standard serological methods using alloantisera and one-dimensional isoelectric focusing are used for the typing of HLA class I antigens. Recently, DNA analysis of class I alleles has been introduced. In HLA class II typing the serological analysis was generally replaced by DNA analysis. In addition to typing techniques that determine the individual HLA alleles or HLA gene products, the cellular matching techniques are used in the selection procedure (mixed lymphocyte culture, cytotoxic T lymphocyte precursor frequency assay, and IL-2-producing helper T lymphocyte precursor frequency assay). The cellular matching techniques determine the compatibility in the regions of HLA comprising several HLA loci; some of them may detect minor histocompatibility (non-HLA) gene disparities as well.
- MeSH
- dárci tkání * MeSH
- HLA antigeny MeSH
- lidé MeSH
- testování histokompatibility MeSH
- transplantace kostní dřeně MeSH
- výběr pacientů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- HLA antigeny MeSH
