The CD94 receptor, expressed on natural killer (NK) and CD8+ T cells, is known as a relatively non-polymorphic receptor with orthologues in humans, other primates, cattle, and rodents. In the house mouse (Mus musculus), a single allele is highly conserved among laboratory strains, and reports of allelic variation in lab- or wild-living mice are lacking, except for deficiency in one lab strain (DBA/2J). The non-classical MHC-I molecule Qa-1b is the ligand for mouse CD94/NKG2A, presenting alternative non-americ fragment of leader peptides (Qa-1 determinant modifier (Qdm)) from classical MHC-I molecules. Here, we report a novel allele identified in free-living house mice captured in Norway, living among individuals carrying the canonical Cd94 allele. The novel Cd94LocA allele encodes 12 amino acid substitutions in the extracellular lectin-like domain. Flow cytometric analysis of primary NK cells and transfected cells indicates that the substitutions prevent binding of CD94 mAb and Qa-1b/Qdm tetramers. Our data further indicate correlation of Cd94 polymorphism with the two major subspecies of house mice in Europe. Together, these findings suggest that the Cd94LocA/NKG2A heterodimeric receptor is widely expressed among M. musculus subspecies musculus, with ligand-binding properties different from mice of subspecies domesticus, such as the C57BL/6 strain.
- Klíčová slova
- Comparative immunology/evolution, Genomics, Inhibitory/activating receptors, MHC, NK cell,
- MeSH
- alely MeSH
- buňky NK metabolismus MeSH
- CD8-pozitivní T-lymfocyty metabolismus MeSH
- CHO buňky MeSH
- Cricetulus MeSH
- druhová specificita MeSH
- HEK293 buňky MeSH
- histokompatibilita - antigeny třídy I chemie genetika metabolismus MeSH
- křečci praví MeSH
- lektinové receptory NK-buněk - podrodina C chemie genetika metabolismus MeSH
- lektinové receptory NK-buněk - podrodina D chemie genetika metabolismus MeSH
- lidé MeSH
- multimerizace proteinu MeSH
- myši inbrední C57BL MeSH
- myši inbrední DBA MeSH
- peptidy chemie genetika metabolismus MeSH
- polymorfismus genetický * MeSH
- sekvence aminokyselin MeSH
- sekvenční homologie aminokyselin MeSH
- vazba proteinů MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Norsko MeSH
- Názvy látek
- histokompatibilita - antigeny třídy I MeSH
- lektinové receptory NK-buněk - podrodina C MeSH
- lektinové receptory NK-buněk - podrodina D MeSH
- peptidy MeSH
- Q surface antigens MeSH Prohlížeč
- Qdm protein, mouse MeSH Prohlížeč
