Nitric oxide (NO) plays a key role in many physiological processes in plants, including pollen tube growth. Here, effects of NO on extracellular Ca(2+) flux and microfilaments during cell wall construction in Pinus bungeana pollen tubes were investigated. Extracellular Ca(2+) influx, the intracellular Ca(2+) gradient, patterns of actin organization, vesicle trafficking and cell wall deposition upon treatment with the NO donor S-nitroso-N-acetylpenicillamine (SNAP), the NO synthase (NOS) inhibitor N(omega)-nitro-L-arginine (L-NNA) or the NO scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were analyzed. SNAP enhanced pollen tube growth in a dose-dependent manner, while L-NNA and cPTIO inhibited NO production and arrested pollen tube growth. Noninvasive detection and microinjection of a Ca(2+) indicator revealed that SNAP promoted extracellular Ca(2+) influx and increased the steepness of the tip-focused Ca(2+) gradient, while cPTIO and L-NNA had the opposite effect. Fluorescence labeling indicated that SNAP, cPTIO and L-NNA altered actin organization, which subsequently affected vesicle trafficking. Finally, the configuration and/or distribution of cell wall components such as pectins and callose were significantly altered in response to L-NNA. Fourier transform infrared (FTIR) microspectroscopy confirmed the changes in the chemical composition of walls. Our results indicate that NO affects the configuration and distribution of cell wall components in pollen tubes by altering extracellular Ca(2+) influx and F-actin organization.
- MeSH
- barvení a značení MeSH
- benzoáty farmakologie MeSH
- biologické modely MeSH
- borovice účinky léků metabolismus MeSH
- buněčná stěna účinky léků metabolismus MeSH
- časové faktory MeSH
- extracelulární prostor účinky léků metabolismus MeSH
- glukany metabolismus MeSH
- imidazoly farmakologie MeSH
- intracelulární prostor účinky léků metabolismus MeSH
- klíčení účinky léků MeSH
- kvartérní amoniové sloučeniny metabolismus MeSH
- mikrofilamenta účinky léků metabolismus MeSH
- nitroarginin farmakologie MeSH
- oxid dusnatý biosyntéza farmakologie MeSH
- pektiny metabolismus MeSH
- polymerizace účinky léků MeSH
- pylová láčka cytologie účinky léků růst a vývoj metabolismus MeSH
- pyridinové sloučeniny metabolismus MeSH
- S-nitroso-N-acetylpenicilamin farmakologie MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- vápník metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole MeSH Prohlížeč
- benzoáty MeSH
- callose MeSH Prohlížeč
- FM 4-64 MeSH Prohlížeč
- glukany MeSH
- imidazoly MeSH
- kvartérní amoniové sloučeniny MeSH
- nitroarginin MeSH
- oxid dusnatý MeSH
- pektiny MeSH
- pyridinové sloučeniny MeSH
- S-nitroso-N-acetylpenicilamin MeSH
- vápník MeSH