OBJECTIVE: A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS: The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-kappa. VH3-9 region of IgVH genes was rearranged and somatically hypermutated. The UHKT-944 cells were found to be negative for most of tested B-cell, T-cell, and myeloid markers. According to cytogenetic analysis, the cells were classified as near tetraploid with several numerical and structural abnormalities including the t(14;20) involving IgH locus. CONCLUSION: The established permanent plasma cell leukemia cell line is a suitable model for the study of cellular and molecular mechanisms of pathogenesis of this rare malignant disease.
- Klíčová slova
- human plasma cell line, interleukin-6 (IL-6), multiple myeloma, plasma cell, plasma cell leukemia,
- MeSH
- biologické markery MeSH
- cytogenetické vyšetření MeSH
- imunofenotypizace MeSH
- imunoglobuliny genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- plazmocelulární leukemie diagnóza metabolismus patologie MeSH
- proliferace buněk MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické markery MeSH
- imunoglobuliny MeSH
