The impact of transgenic white poplars (Populus alba L. cv. 'Villafranca') was assessed on the soil aerobic spore-forming bacteria (SFB). The genetically modified poplars, expressing either the StSy gene for resveratrol production or the bar gene for herbicide tolerance, were cultivated in greenhouse. The occurrence of SFB was monitored in soil samples collected at eight different timepoints over a two-year period. The total culturable bacterial population of the StSy and bar trials underwent significant seasonal fluctuations in the range of 10(6)-2.5 x 10(8) CFU/g dry soil and of 10(4)-5 x 10(8) CFU/g dry soil, respectively. Changes occurred also within the culturable SFB population with size varying at 10(3)-5 x 10(4) CFU/g dry soil and 10(2)-2 x 10(5) CFU/g dry soil in the StSy and bar trials, respectively. No significant differences in the size of the total and SFB culturable populations were observed when comparing each transgenic line with the nontransformed control line while seasonal shifts of soil bacterial populations were evident in both trials. The culturable SFB fraction included three isolates (SFB-1, SFB-2 and SFB-3) classified by 16S rDNA sequence analysis as members of the Bacillus genus. According to the reported data, cultivation of both herbicide-resistant and resveratrol-producing GM white poplars did not affect the culturable SFB population at the soil level.

• MeSH
• Aerobiosis MeSH
• Endospore-Forming Bacteria classification   isolation & purification   physiology   MeSH
• DNA, Bacterial chemistry   genetics   MeSH
• Phylogeny MeSH
• Plants, Genetically Modified microbiology   MeSH
• Molecular Sequence Data MeSH
• Colony Count, Microbial MeSH
• Populus microbiology   MeSH
• Soil Microbiology * MeSH
• DNA, Ribosomal chemistry   genetics   MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• Seasons MeSH
• Sequence Analysis, DNA MeSH
• Cluster Analysis MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA, Bacterial MeSH
• DNA, Ribosomal MeSH
• RNA, Ribosomal, 16S MeSH

In 2001, a Gram-variable, facultatively anaerobic, endospore-forming bacterium isolated from biodeteriorated mural paintings in the Servilia tomb of the Roman necropolis of Carmona was deposited as Paenibacillus strain LMG 19508. Subsequently, the strain was characterized in detail using phenotypic and molecular methods. The 16S rRNA gene sequence confirmed that the strain belongs to the genus Paenibacillus and indicated its relationship to Paenibacillus mendelii CCM 4839(T) (96.7 % sequence similarity). The predominant menaquinone was MK-7. The cell wall contained meso-diaminopimelic acid of the A1gamma type. The DNA G+C content (50 mol%) and the major fatty acid (anteiso-C(15 : 0)) of strain LMG 19508(T) were also consistent with its affiliation to the genus Paenibacillus. DNA-DNA hybridization distinguished strain LMG 19508(T) from other phylogenetically related Paenibacillus species. Therefore, the isolate represents a novel species, for which the name Paenibacillus sepulcri sp. nov. is proposed. The type strain is CCM 7311(T) (=LMG 19508(T)).

• MeSH
• Bacteria, Anaerobic classification   genetics   isolation & purification   physiology   MeSH
• Endospore-Forming Bacteria classification   genetics   isolation & purification   physiology   MeSH
• DNA, Bacterial analysis   MeSH
• Phenotype MeSH
• Phylogeny MeSH
• Genotype MeSH
• Genes, rRNA MeSH
• Paintings * MeSH
• Fatty Acids analysis   MeSH
• Molecular Sequence Data MeSH
• Burial MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• Sequence Analysis, DNA MeSH
• Spores, Bacterial physiology   MeSH
• Bacterial Typing Techniques MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Spain MeSH
• Names of Substances
• DNA, Bacterial MeSH
• Fatty Acids MeSH
• RNA, Ribosomal, 16S MeSH