The circulating antibody profiles of rabbits infected or immunized with Sarcoptes scabiei var. canis were compared. Crossed immuno-electrophoretic analysis showed that infested hosts produced serum antibody to 12 proteins (antigens) in an extract made from sarcoptic mite bodies. In contrast, rabbits immunized with an extract made from mite bodies produced antibody to 20 Sarcoptes proteins (antigens). SDS-PAGE/immunoblot analysis revealed that serum from immunized rabbits contained antibodies that bound strongly to proteins of 25 and 39-52 kD that were only barely visualized by antibodies in serum from infested rabbits.

• MeSH
• Antigens analysis   MeSH
• Immunoelectrophoresis, Two-Dimensional MeSH
• Rabbits MeSH
• Antibodies blood   MeSH
• Sarcoptes scabiei immunology   MeSH
• Scabies immunology   MeSH
• Tissue Extracts immunology   MeSH
• Vaccination MeSH
• Animals MeSH
• Check Tag
• Rabbits MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH
• Comparative Study MeSH
• Names of Substances
• Antigens MeSH
• Antibodies MeSH
• Tissue Extracts MeSH

• MeSH
• Sarcoptes scabiei ultrastructure   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH

All the above listed reasons as well as the experience we have obtained with this method, justify our recommendation of the introduction of the laboratory diagnosis of scabies through the method of lye scraping of the skin into routine practice. A single parasite element is enough for determining the diagnosis while, for the above mentioned reasons, a negative microscope finding does not exclude the parasitic etiology of the given disease. We therefore recommend in the diagnosis of scabies that the following criteria are parallely used: 1. an objective skin finding 2. laboratory proof of the causative agent of infestation 3. subjective feelings of the patient (itching at the typical time) 4. positive epidemiological anamnesis, primarily the occurrence of clinical symptoms in several members of a family or a group 5. diminishing of clinical symptoms after specific treatment with an anti-scabies medicament.

• MeSH
• Adult MeSH
• Skin parasitology   MeSH
• Humans MeSH
• Lye MeSH
• Methods MeSH
• Child, Preschool MeSH
• Sarcoptes scabiei isolation & purification   MeSH
• Scabies diagnosis   parasitology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Male MeSH
• Child, Preschool MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Lye MeSH

• MeSH
• Humans MeSH
• Bedding and Linens * MeSH
• Dust MeSH
• Mites isolation & purification   MeSH
• Sarcoptes scabiei isolation & purification   MeSH
• Scabies etiology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Dust MeSH

• MeSH
• Caustics MeSH
• Humans MeSH
• Methods MeSH
• Solutions MeSH
• Sarcoptes scabiei isolation & purification   MeSH
• Scabies diagnosis   epidemiology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Czechoslovakia MeSH
• Names of Substances
• Caustics MeSH
• Solutions MeSH

• MeSH
• Humans MeSH
• Sarcoptes scabiei growth & development   MeSH
• Scabies microbiology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH