The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore an acapsular strain was used. The following actin structures were found: actin dots, actin cables and cytokinetic ring. Ultrastructural studies showed the presence of a nucleus in the bud before mitosis. A collar-shaped structure was seen at the base of bud emergence. A lamellar cell wall and a rough outer surface of the cells were detected. Cytoskeletal structures found in C. laurentii are similar to those in Cryptococcus neoformans, which is a serious human pathogen.
- MeSH
- aktiny metabolismus MeSH
- biologické modely MeSH
- buněčná stěna metabolismus MeSH
- buněčné jádro metabolismus ultrastruktura MeSH
- Cryptococcus metabolismus ultrastruktura MeSH
- cytoskelet metabolismus MeSH
- faloidin analogy a deriváty chemie MeSH
- fluorescenční mikroskopie MeSH
- mikrotubuly metabolismus MeSH
- mitóza MeSH
- rhodaminy chemie MeSH
- transmisní elektronová mikroskopie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aktiny MeSH
- faloidin MeSH
- rhodamine-phalloidin MeSH Prohlížeč
- rhodaminy MeSH
