In situ extraction of steroid hormones from waste water using adsorption-based integrative passive samplers represents a promising approach for their monitoring in water at ultra-trace concentrations. Three passive samplers, namely a POCIS, a Chemcatcher fitted with an Empore SDB-RPS disk, and an Empore SDB-RPS disk-based sampler with enhanced water flow, were calibrated in situ in treated municipal wastewater for the purpose of monitoring five estrogens (17-β-estradiol, 17-α-estradiol, 17-α-ethinylestradiol, estrone and estriol) at sub ng per litre concentrations. Uptake of steroids to samplers during 14-day exposure in wastewater was compared with steroid concentrations in daily collected composite water samples. Sampling rates were obtained from a numerical solution of first order uptake kinetics equations describing the uptake of compounds into a passive sampler over time. Mass transfer of steroids in the Chemcatcher fitted with naked Empore disks was more than two times faster than in the POCIS sampler. The uptake capacity of the applied Empore disk was not sufficient for integrative uptake of all tested steroids during the entire 14-day exposure. Time-weighted average concentrations of steroids estimated at concentrations in units of ngL-1 using the in situ-calibrated samplers were within a factor of two from values obtained using composite water samples.

• Klíčová slova
• Chemcatcher, POCIS, Passive sampling, Steroid hormones, Waste water, Water monitoring,
• MeSH
• chemické látky znečišťující vodu analýza   MeSH
• estrogeny analýza   MeSH
• kalibrace MeSH
• kongenery estradiolu analýza   MeSH
• monitorování životního prostředí přístrojové vybavení   MeSH
• odpadní voda analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• estrogeny MeSH
• kongenery estradiolu MeSH
• odpadní voda MeSH

A fast and sensitive analytical procedure has been developed for the simultaneous separation and determination of alpha-estradiol, beta-estradiol, estriol, estrone and ethynylestradiol and their sulfate, glucuronide and acetate conjugates in river sediments. The procedure includes a microwave-assisted extraction (MAE) with aqueous methanol (25:75, v/v) at 100 degrees C in 10 min, a clean-up on Oasis WAX cartridge and a high-performance liquid chromatography-ion-trap tandem mass spectrometry (HPLC-IT-MS/MS) with electrospray ionization. The recovery for each compounds ranged from 83 to 107% and the repeatability represented as RSDs ranged from 4.9 to 9.6%. The limits of detection (LODs) were down to 1 ng g(-1). The analytical performance of the method was evaluated using determination of free and conjugated estrogens in river sediments.

• MeSH
• chemické látky znečišťující vodu izolace a purifikace   MeSH
• chromatografie iontoměničová metody   MeSH
• estrogeny analýza   chemie   MeSH
• extrakce na pevné fázi metody   MeSH
• geologické sedimenty chemie   MeSH
• hmotnostní spektrometrie s elektrosprejovou ionizací metody   MeSH
• kongenery estradiolu analýza   chemie   MeSH
• látky znečišťující půdu analýza   chemie   MeSH
• mikrovlny MeSH
• monitorování životního prostředí metody   MeSH
• řeky chemie   MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• estrogeny MeSH
• kongenery estradiolu MeSH
• látky znečišťující půdu MeSH