A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively. To confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic DNA (McRAPD). Further, the ability to form biofilm and its susceptibility to systemic antifungals were evaluated. Using McRAPD, identity of C. lusitaniae isolates showing slight microevolutionary changes in karyotypes was undoubtedly confirmed; successful application of numerical interpretation of McRAPD for typing is demonstrated here for the first time. The strain was also recognized as a strong biofilm producer. Moreover, minimum biofilm inhibitory concentrations were very high, in contrast to low antifungal minimum inhibitory concentrations of isolates. It can be concluded that McRAPD seems to be a simple and reliable method not only for identification but also for typing of yeasts. A ventriculoperitoneal shunt colonized by C. lusitaniae was revealed as the source of this nosocomial infection, and the ability of the strain to form biofilm on its surface likely caused treatment failure.

• MeSH
• antifungální látky farmakologie   MeSH
• astrocytom mozkomíšní mok   komplikace   farmakoterapie   mikrobiologie   patologie   chirurgie   MeSH
• biofilmy účinky léků   růst a vývoj   MeSH
• Candida účinky léků   genetika   izolace a purifikace   MeSH
• denaturace nukleových kyselin MeSH
• dítě MeSH
• hydrocefalus mozkomíšní mok   komplikace   farmakoterapie   mikrobiologie   patologie   chirurgie   MeSH
• infekce spojené se zdravotní péčí mozkomíšní mok   komplikace   farmakoterapie   mikrobiologie   patologie   chirurgie   MeSH
• kandidóza mozkomíšní mok   komplikace   farmakoterapie   mikrobiologie   patologie   chirurgie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• mykologické určovací techniky MeSH
• nádory mozku mozkomíšní mok   komplikace   farmakoterapie   mikrobiologie   patologie   chirurgie   MeSH
• neúspěšná terapie MeSH
• pulzní gelová elektroforéza MeSH
• technika náhodné amplifikace polymorfní DNA * MeSH
• ventrikuloperitoneální zkrat škodlivé účinky   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifungální látky MeSH

In the period from November 1998 to June 2001 13 cases of nosocomial meningitis were reported. Candida albicans was isolated from 54% of the patients (7); C. parapsilosis from 23% (3); C. tropicalis from 15% (2) and C. krusei from 8% (1). C. albicans was isolated from the cerebrospinal fluid (CSF) of five children with the following diagnoses: nonspecified tumor of the central nervous system, Hodgkin's disease, meningitis, suspect neuroinfection, and sepsis. Examination of CSF allowed us to detect 2 strains of C. albicans from adult patients, one after neurosurgery because of a brain tumor and one with a vascular disease of the brain. C. parapsilosis was found in CSF from two premature children and one child with epilepsy. Two isolates of C. tropicalis were obtained from both blood and the CSF of a child from the neonatal intensive care unit and from a child from pediatric oncology with multiple malignant neoplasms. Only one strain of C. krusei was found in the oral cavity and CSF of a patient after neurosurgery performed after head trauma.

• MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• dítě MeSH
• dospělí MeSH
• fungální meningitida mozkomíšní mok   mikrobiologie   MeSH
• jednotky intenzivní péče o novorozence MeSH
• kandidóza mozkomíšní mok   epidemiologie   mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mozkomíšní mok mikrobiologie   MeSH
• novorozenec MeSH
• rizikové faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH