Occurrence of Candida spp. was determined in a population of 60 infants, 1-15-month-old, with diaper dermatitis, admitted to a neonatal intensive care unit in Hospital Saca (Kosice, Slovakia). Specimens were obtained from the perianal, pubic, inguinal, or gluteal areas that showed signs of secondary infection as manifested by erythema, oozing, vesiculopustular lesions, and pus formation. The most frequently isolated species was C. albicans (41), followed by C. parapsilosis (8), C. tropicalis (4), C. pulcherrima (4), C. guilliermondii (2), and C. zeylanoides (1). Other organisms present in the mixed culture from the diaper area were Staphylococcus aureus (6), Escherichia coli (3), and 2 strains of each group B and D streptococci, and Proteus mirabilis. Infants diapered exclusively in disposable diapers showed less rash than those diapered exclusively or sometimes in cloth diapers.

• MeSH
• Candida izolace a purifikace   MeSH
• kandidóza kožní mikrobiologie   patologie   MeSH
• kojenec MeSH
• lidé MeSH
• plenková dermatitida mikrobiologie   patologie   MeSH
• pleny dětské mikrobiologie   MeSH
• průjem kojenců mikrobiologie   patologie   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The incidence of non-albicans Candida and non-Candida species isolated from the urine of patients admitted to various departments of the Faculty Hospital of the Medical Faculty of Safárik University in Kosice was examined. From a total of 94 samples of analyzed urine 58 strains of C. albicans and 36 strains of yeasts belonging to 6 species of non-albicans Candida and non-Candida spp. were detected: C. parapsilosis (n = 23), C. tropicalis (6), C. krusei (3), C. robusta (2), C. catenulata (1) and Cryptococcus neoformans (1). In relation to the diagnosis, the yeasts were isolated from patients suffering from a kidneys disease, epididymitis, diabetes, neoplastic diseases, urogenital anomalies, obstructive uropathy, cystitis, prostatitis, hemolytic-uremic syndrome, and others.

• MeSH
• Candida klasifikace   růst a vývoj   izolace a purifikace   MeSH
• infekce močového ústrojí mikrobiologie   MeSH
• kandidóza komplikace   mikrobiologie   MeSH
• lidé MeSH
• nemoci novorozenců MeSH
• novorozenec MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

In the period from November 1998 to June 2001 13 cases of nosocomial meningitis were reported. Candida albicans was isolated from 54% of the patients (7); C. parapsilosis from 23% (3); C. tropicalis from 15% (2) and C. krusei from 8% (1). C. albicans was isolated from the cerebrospinal fluid (CSF) of five children with the following diagnoses: nonspecified tumor of the central nervous system, Hodgkin's disease, meningitis, suspect neuroinfection, and sepsis. Examination of CSF allowed us to detect 2 strains of C. albicans from adult patients, one after neurosurgery because of a brain tumor and one with a vascular disease of the brain. C. parapsilosis was found in CSF from two premature children and one child with epilepsy. Two isolates of C. tropicalis were obtained from both blood and the CSF of a child from the neonatal intensive care unit and from a child from pediatric oncology with multiple malignant neoplasms. Only one strain of C. krusei was found in the oral cavity and CSF of a patient after neurosurgery performed after head trauma.

• MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• dítě MeSH
• dospělí MeSH
• fungální meningitida mozkomíšní mok   mikrobiologie   MeSH
• jednotky intenzivní péče o novorozence MeSH
• kandidóza mozkomíšní mok   epidemiologie   mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mozkomíšní mok mikrobiologie   MeSH
• novorozenec MeSH
• rizikové faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

An indirect immunofluorescence assay was performed to detect antibodies to Candida albicans blastospores and germ tubes. Serum specimens were obtained from 82 patients with neoplastic diseases in the orofacial region and thrush of the oral mucosa. C. albicans was identified in the oral cavity of 63 patients investigated but serum anti-Candida antibodies were detected in only 23 of them. Serological examination showed that titers of antibodies to C. albicans blastospores ranged from 1:20 to 1:1280. High titers from 1:640 to 1:1280 were detected in patients without antibiotic, cytostatic, or radiotherapeutic treatment. The titers of antibodies to C. albicans germ tubes ranged from 1:20 to 1:640. Our results indicate that titers of antibodies to the C. albicans germ tubes were lower and were detected in a smaller number of patients.

• MeSH
• Candida albicans imunologie   izolace a purifikace   MeSH
• fluorescenční protilátková technika nepřímá MeSH
• kandidóza komplikace   imunologie   mikrobiologie   MeSH
• karcinom krev   komplikace   mikrobiologie   MeSH
• lidé MeSH
• nádory úst krev   komplikace   mikrobiologie   MeSH
• orální kandidóza komplikace   imunologie   mikrobiologie   MeSH
• protilátky fungální krev   MeSH
• ústní sliznice mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky fungální MeSH

The proportion of Candida and non-Candida species in the clinical material from patients with respiratory-tract diseases was determined. C. albicans was isolated in 102 cases. An additional 89 strains of yeasts, isolated in association with respiratory diseases, belonged to 10 non-albicans Candida spp. and Cryptococcus spp. The prevailing species, which occurred in 47 cases, was C. parapsilosis. C. tropicalis, C. glabrata, and C. guilliermondii were isolated in 12, 10, and 9 cases, respectively. Four strains of C. krusei and three strains of C. lusitaniae and one strain each of C. freyschussii, C. robusta, C. zeylanoides, and Cryptococcus neoformans were also isolated.

• MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• Cryptococcus izolace a purifikace   MeSH
• infekce dýchací soustavy mikrobiologie   MeSH
• kandidóza epidemiologie   mikrobiologie   MeSH
• lidé MeSH
• nemoci nedonošenců epidemiologie   mikrobiologie   MeSH
• novorozenec MeSH
• sputum mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Anti-Candida antibodies were determined in a group of preterm neonates from a neonatal intensive care unit with serious diseases including candidemia. Antibodies to C. albicans blastospores, i.e. antibodies to C. albicans surface mannan and to C. albicans germ tubes were detected. Higher titers of antibodies to blastospores (1:320) occurred in all patients examined while antibodies to C. albicans germ tubes (with the highest titer of 1:160) were present in 32 out of 66 neonates examined. The highest titers of both anti-C. albicans blastospore antibodies and anti-C. albicans germ tube antibodies were detected in neonates with candidemia and disorders of saccharide metabolism.

• MeSH
• Candida albicans růst a vývoj   imunologie   izolace a purifikace   MeSH
• jednotky intenzivní péče o novorozence statistika a číselné údaje   MeSH
• kandidóza krev   diagnóza   imunologie   MeSH
• lidé MeSH
• nemoci nedonošenců epidemiologie   MeSH
• novorozenec MeSH
• prevalence MeSH
• protilátky fungální krev   MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky fungální MeSH

Candida-associated denture stomatitis was demonstrated by its cultivation in 171 out of 240 patients examined with partial or total dentures. After taking smears from lesions of the oral mucosa (tongue, cheeks, palate) and the contiguous denture surface by cotton wool swabs and inoculating them onto Sabouraud glucose agar and CHROMagar Candida, individual yeast species were identified by a germ tube, filamentous, and assimilation tests employing the commercial kit AuxaColor. Seven Candida species were identified in smears from the oral mucosa lesions and the contiguous denture surface: C. albicans (95 patients), C. tropicalis (26), C. parapsilosis (20), C. krusei (14), C. guilliermondii (12), C. lusitaniae (1) and C. freyschusii (1). Diabetes mellitus, neoplastic diseases, chemotherapy, radiotherapy, and broad-spectrum antibiotic therapy were identified as some of the large number of factors predisposing patients to stomatitis prothetica.

• MeSH
• Candida izolace a purifikace   MeSH
• druhová specificita MeSH
• jazyk mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• orální kandidóza mikrobiologie   MeSH
• patro mikrobiologie   MeSH
• senioři MeSH
• stomatitida vyvolaná protézou mikrobiologie   MeSH
• tvář mikrobiologie   MeSH
• ústní sliznice mikrobiologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The proportion of yeast species involved in eye infections in 11 patients was examined. The presence of yeast organisms as causative agents of endophthalmitis was found in corneal smears (n = 4), conjunctival swabs (4), and vitreous fluid (3). Altogether 5 strains of Candida albicans, 2 strains of C. krusei and one strain each of C. guilliermondii, C. parapsilosis, C. tropicalis and Cryptococcus neoformans were isolated from the clinical material. The hematogenic origin of endophthalmitis was proved in 7 cases on the basis of positive blood samples and in 2 cases by the isolation of yeasts from the tip of an intravenous catheter. Endophthalmitis-supporting risk factors such as indwelling intravenous catheters, prolonged use of broad-spectrum antibiotics and chemotherapy, surgical intervention, diabetes mellitus, and malignancy were observed in the patients.

• MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• Cryptococcus neoformans izolace a purifikace   MeSH
• kandidóza mikrobiologie   MeSH
• kryptokokóza mikrobiologie   MeSH
• kvasinky izolace a purifikace   MeSH
• lidé MeSH
• novorozenec MeSH
• oční infekce mykotické mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH

Electrophoretic karyotyping was used to compare DNA probes of yeasts isolated from blood of preterm neonates (n = 66) in a neonatal intensive care unit (NICU) and from the hands of healthy hospital personnel (n = 10). The yeasts were identified as Candida albicans using standard laboratory methods. DNA was extracted from yeasts and isolation of identical DNA strains from the pairs nurse-neonate suggested that one nurse transmitted one yeast strain by her hands to three neonates. Four neonates harbored two identical strains originating from two nurses, i.e. each nurse transmitted the same strain to two neonates. In the additional 7 cases transmission of 1 yeast strain by 1 nurse to 1 neonate was observed. Our data suggest that nonperinatal nosocomial transmission of C. albicans occurs in neonates, possibly via cross-contamination being transferred on hands of health care workers. The importance of careful hand washing of staff (health care workers) and other infection-control procedures (to prevent the nosocomial transmission of pathogens in the NICU environment) is emphasized.

• MeSH
• Candida albicans genetika   izolace a purifikace   MeSH
• fungemie mikrobiologie   MeSH
• infekce spojené se zdravotní péčí mikrobiologie   přenos   MeSH
• jednotky intenzivní péče o novorozence * MeSH
• kandidóza epidemiologie   mikrobiologie   přenos   MeSH
• karyotypizace MeSH
• lidé MeSH
• novorozenec nedonošený krev   MeSH
• novorozenec MeSH
• personál nemocniční * MeSH
• přenos infekce ze zdravotnického pracovníka na pacienta MeSH
• prospektivní studie MeSH
• ruka MeSH
• zdravotní sestry MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Geografické názvy
• Slovenská republika epidemiologie   MeSH

Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and Grocott-positive elements were exclusively observed in the superficial epithelial layers, e.g., the cornified layer and the stratum granulosum. The inflammatory reaction was characterized by an early phase during which polymorphonuclear cells predominated. The skeletal muscle fascicles immediately adjacent to the infected epithelium showed striking degeneration and atrophy associated with a marked infiltrate of chronic inflammatory cells.

• MeSH
• atrofie patologie   MeSH
• barvení a značení MeSH
• biopsie MeSH
• Candida izolace a purifikace   MeSH
• hyfy izolace a purifikace   MeSH
• kosterní svaly patologie   MeSH
• kyselina jodistá MeSH
• lidé středního věku MeSH
• lidé MeSH
• orální kandidóza mikrobiologie   patologie   MeSH
• orální leukoplakie mikrobiologie   patologie   MeSH
• senioři MeSH
• ústní sliznice mikrobiologie   patologie   MeSH
• zánět patologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kyselina jodistá MeSH