The ability of C. parapsilosis (an important cause of nosocomial infections) to produce biofilm was evaluated in 32 bloodstream isolates and 85 strains isolated from skin. The biofilm formation was found in 19 (59%) blood isolates and only in 33 (39%) isolates from skin. The antifungal susceptibility was assessed for amphotericin B, itraconazole and voriconazole in planktonic and biofilm form of the 19 biofilm-positive bloodstream strains by broth microdilution method according to NCCLS standards. The method was modified by the use of resazurin as a colorimetric indicator of the metabolically active cells which makes the determination of the effect of antifungal agents easier. Biofilm forms of all strains were more resistant than their planktonic form.

• MeSH
• antifungální látky farmakologie   MeSH
• biofilmy účinky léků   MeSH
• Candida účinky léků   patogenita   MeSH
• fungální léková rezistence účinky léků   MeSH
• fungemie farmakoterapie   MeSH
• infekce spojené se zdravotní péčí mikrobiologie   MeSH
• kolorimetrie MeSH
• lidé MeSH
• mikrobiální testy citlivosti metody   MeSH
• mikrobiologické techniky MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifungální látky MeSH

Distribution of Candida species was investigated by examining 245 samples from skin lesions and nails. The isolates were identified using standard laboratory methods including germ tube test, micromorphology of colonies on rice agar, the commercial kit, saccharide assimilation and fermentation tests. Eight species of Candida were identified: C. albicans accounted for 56.4% of the isolates, C. parapsilosis 29.1, C. tropicalis 7.8, C. pulcherrima 2.9, C. guilliermondii 1.5, C. krusei and C. zeylanoides for 0.9% each, and C. robusta for 0.5%. The factors significantly associated with colonization were prolonged antibiotic therapy, parenteral nutrition, low birth body mass of infants, intubation, duration of stay in hospital, indwelling intravenous catheter, malignancies, diabetes, surgery, and obesity.

• MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• dermatologie MeSH
• dospělí MeSH
• incidence MeSH
• jednotky intenzivní péče o novorozence MeSH
• kandidóza kožní epidemiologie   mikrobiologie   MeSH
• kandidóza epidemiologie   mikrobiologie   MeSH
• lidé MeSH
• mykologické určovací techniky MeSH
• nehty mikrobiologie   MeSH
• nemoci nehtů epidemiologie   mikrobiologie   MeSH
• novorozenec MeSH
• rizikové faktory MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH