Viral CC chemokine inhibitor (vCCI) of the clone P13 vaccinia virus (VACV) strain PRAHA lacks eight amino acids in the signal peptide sequence. To study the influence of vCCI on virus biology, a virus with the vCCI gene coding for a prolonged signal sequence was prepared. We found that secreted vCCI attenuated the virus in vivo, and that it correlated with decreased levels of RANTES, eotaxin, TARC, and MDC in the blood in comparison with the parental virus. We determined the influence of vCCI on the CTL response against VACV E3((140-148)) (VGPSNSPTF) and HPV16 E7((49-57)) (RAHYNIVTF) H-2D(b)-restricted epitopes. The examination of the specific CTL response elicited by immunization with the recombinant VACV-expressing tumor-associated HPV16 E7 antigen by IFN-γ ELISPOT showed that the immunogenicity of the recombinant VACV-producing secretory vCCI was similar to that of the parent virus or deletion mutant in the C23L/B29R locus. Immunization with the secretory vCCI-producing recombinant virus has a lower therapeutic anti-tumor effect against TC-1 tumors. Viral CCI downregulated the E7-specific response induced by gene gun immunization with the DNA vaccines pBSC-SigE7 LAMP and pBSC-vCCI. We also observed that the immune response against vCCI elicited by the DNA vaccine did not affect the multiplication of VACV in vivo.

• MeSH
• Cell Line MeSH
• Chemokine CCL17 blood   MeSH
• Chemokine CCL5 blood   MeSH
• Chemokines, CC antagonists & inhibitors   blood   MeSH
• T-Lymphocytes, Cytotoxic immunology   MeSH
• Mice, Inbred C57BL MeSH
• Mice MeSH
• Tumor Suppressor Proteins blood   MeSH
• Papillomavirus E7 Proteins genetics   immunology   MeSH
• ADAM Proteins blood   MeSH
• Cancer Vaccines genetics   immunology   MeSH
• Sequence Deletion MeSH
• Vaccines, Synthetic genetics   immunology   MeSH
• Vaccination MeSH
• Viral Proteins genetics   metabolism   MeSH
• Viral Vaccines immunology   MeSH
• Vaccinia virus genetics   immunology   pathogenicity   MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• ADAM11 protein, human MeSH Browser
• Chemokine CCL17 MeSH
• Chemokine CCL5 MeSH
• Chemokines, CC MeSH
• Tumor Suppressor Proteins MeSH
• oncogene protein E7, Human papillomavirus type 16 MeSH Browser
• Papillomavirus E7 Proteins MeSH
• ADAM Proteins MeSH
• Cancer Vaccines MeSH
• Vaccines, Synthetic MeSH
• Viral Proteins MeSH
• Viral Vaccines MeSH