Under electron microscope, the matrix of sectioned mitochondria exhibits ribosomes and an oval, electron-transparent zone which is devoid of ribosomes and is named chondriolite. Fine fibers or clumps of an electron-dense material appeared in this zone after several fixation and contrasting steps and were identified with mitochondrial DNA by cytologists. To verify this assumption, we labeled DNA by a monoclonal antibody and a secondary antibody coupled to immunogold. The label was observed in the nucleus and in the chondriolite zone of sectioned mitochondria. Because the ultrastructure of chondriolites resembles that of nucleoids of prokaryotes, we suggest the term mitochondrial nucleoid for the zone of mitochondrial matrix devoid of ribosomes and containing DNA.

• MeSH
• aspartátkarbamoyltransferasa analýza   MeSH
• DNA fungální analýza   MeSH
• imunoelektronová mikroskopie metody   MeSH
• karbamoylfosfátsynthasa (hydrolyzující glutamin) analýza   MeSH
• mitochondriální DNA analýza   MeSH
• mitochondrie chemie   ultrastruktura   MeSH
• multienzymové komplexy analýza   MeSH
• Saccharomyces cerevisiae - proteiny * MeSH
• Saccharomyces cerevisiae chemie   genetika   ultrastruktura   MeSH
• submitochondriální částice chemie   ultrastruktura   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aspartátkarbamoyltransferasa MeSH
• DNA fungální MeSH
• karbamoylfosfátsynthasa (hydrolyzující glutamin) MeSH
• mitochondriální DNA MeSH
• multienzymové komplexy MeSH
• Saccharomyces cerevisiae - proteiny * MeSH
• URA2 protein, S cerevisiae MeSH Prohlížeč