A multiplex PCR method was developed to characterize the potential variability within the sip gene from bovine isolates of Streptococcus agalactiae. An incomplete sip gene (ncosip) was found in four isolates of S. agalactiae. According to the in silico analysis of the missing region at amino acid level, the N-terminal of surface immunogenic protein (Sip) was found to contain a LysM domain motif; whilst the incomplete Sip (NcoSip) lacked a part of this motif. Immunity elicitation against Sip was confirmed by immunization of mice. This response was partially observed also with NcoSip.

• MeSH
• aminokyselinové motivy MeSH
• antigeny bakteriální chemie   genetika   imunologie   MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• protilátky bakteriální krev   MeSH
• sekvence aminokyselin MeSH
• Streptococcus agalactiae chemie   genetika   imunologie   MeSH
• streptokokové infekce imunologie   mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny bakteriální MeSH
• protilátky bakteriální MeSH
• SIP protein, Streptococcus group B MeSH Prohlížeč

Signal transduction pathways in both prokaryotes and eukaryotes utilize protein phosphorylation as a key regulatory mechanism. Recent studies have proven that eukaryotic-type serine/threonine protein kinases (Hank's type) are widespread in many bacteria, although little is known regarding the cellular processes they control. In this study, we have attempted to establish the role of a single eukaryotic-type protein kinase, StkP of Streptococcus pneumoniae, in bacterial survival. Our results indicate that the expression of StkP is important for the resistance of S. pneumoniae to various stress conditions. To investigate the impact of StkP on this phenotype, we compared the whole-genome expression profiles of the wild-type and DeltastkP mutant strains by microarray technology. This analysis revealed that StkP positively controls the transcription of a set of genes encoding functions involved in cell wall metabolism, pyrimidine biosynthesis, DNA repair, iron uptake, and oxidative stress response. Despite the reduced transformability of the stkP mutant, we found that the competence regulon was derepressed in the stkP mutant under conditions that normally repress natural competence development. Furthermore, the competence regulon was expressed independently of exogenous competence-stimulating peptide. In summary, our studies show that a eukaryotic-type serine/threonine protein kinase functions as a global regulator of gene expression in S. pneumoniae.

• MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• delece genu MeSH
• eukaryotické buňky enzymologie   MeSH
• fenotyp MeSH
• koncentrace vodíkových iontů MeSH
• mikrobiální viabilita účinky léků   genetika   MeSH
• mutace MeSH
• osmotický tlak MeSH
• oxidační stres MeSH
• peroxid vodíku farmakologie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• protein-serin-threoninkinasy genetika   metabolismus   MeSH
• regulace genové exprese u bakterií * MeSH
• sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
• Streptococcus pneumoniae enzymologie   genetika   růst a vývoj   MeSH
• testy genetické komplementace MeSH
• vysoká teplota MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• peroxid vodíku MeSH
• protein-serin-threoninkinasy MeSH