In this study, we focused on comparing the effects of serotonin and its metabolites on the functions of RAW264.7 cells (emphasis on oxidative burst and production of nitric oxide and cytokines), thereby expanding the scope of existing knowledge with advent of novel findings in this field. Changes in production of reactive oxygen species (ROS) by RAW264.7 cells after treatment with serotonin, N-acetylserotonin and melatonin were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all respective compounds were measured using TRAP and amperometrical method. Nitric oxide (NO) production was measured by Griess reagent and inducible NO synthase (iNOS) expression by Western blot. Cytokine production was assessed using the Mouse Cytokine Panel A Array kit and ELISA. We showed that all tested compounds were able to reduce oxidative stress, as well as inhibit production of inflammatory cytokines by macrophages. Of the tested compounds, serotonin and N-acetylserotonin were markedly better antioxidants than melatonin. In comparison, other effects of tested compounds were very similar. It can be concluded that antioxidant capacity of tested compounds is a major advantage in the early stages of inflammation. Since plasma concentrations of N-acetylserotonin and melatonin are lower than serotonin, it can be deduced that serotonin plays a key role in modulation of inflammation and the regulatory functions of immune cells, while also protecting cells against oxidative stress.

• Klíčová slova
• Cytokines, Melatonin, N-acetylserotonin, Nitric oxide, RAW264.7 macrophages, Reactive oxygen species, Serotonin,
• MeSH
• antioxidancia farmakologie   MeSH
• cytokiny metabolismus   MeSH
• makrofágy metabolismus   MeSH
• melatonin farmakologie   MeSH
• myši MeSH
• oxid dusnatý metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• RAW 264.7 buňky MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• serotonin analogy a deriváty   farmakologie   MeSH
• zánět metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• cytokiny MeSH
• melatonin MeSH
• N-acetylserotonin MeSH Prohlížeč
• oxid dusnatý MeSH
• reaktivní formy kyslíku MeSH
• serotonin MeSH