In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

• MeSH
• Albumins pharmacology   MeSH
• Alkyl and Aryl Transferases genetics   MeSH
• Amino Acids, Aromatic biosynthesis   genetics   MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Genes, Bacterial * MeSH
• Drug Resistance, Bacterial genetics   MeSH
• Cell Membrane drug effects   genetics   MeSH
• Cell Wall drug effects   genetics   MeSH
• Edetic Acid pharmacology   MeSH
• Phosphoenolpyruvate metabolism   MeSH
• Cloning, Molecular MeSH
• Complement System Proteins pharmacology   MeSH
• Mutation MeSH
• Conalbumin pharmacology   MeSH
• Plasmids genetics   MeSH
• Salmonella enteritidis drug effects   enzymology   genetics   MeSH
• Salmonella typhimurium drug effects   enzymology   genetics   MeSH
• Oligonucleotide Array Sequence Analysis MeSH
• Serum MeSH
• Genetic Complementation Test MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Albumins MeSH
• Alkyl and Aryl Transferases MeSH
• Amino Acids, Aromatic MeSH
• Anti-Bacterial Agents MeSH
• Edetic Acid MeSH
• Phosphoenolpyruvate MeSH
• Complement System Proteins MeSH
• Conalbumin MeSH
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase MeSH Browser