Primers were designed and prepared and conditions were determined for PCR detection and differentiation of enterotoxigenic E. coli bacterial strains isolated from diarrheic pigs. Primers K88/1 and K88/2 are 25 bp oligomers that correspond to a region of genes encoding one of serological variants of the K88 antigen (K88ab(1), K88ab(2), K88ac or K88ad). A positive result of PCR is an amplificate of 792 bp in size for K88ab and K88ad variant or 786 bp for K88ac variant. The individual serological variants of genes of the K88 antigen could be differentiated by cutting the obtained PCR amplificates by restriction endonucleases. The PCR analysis of 674 E. coli strains isolated from diarrheic pigs showed that 184 strains were K88 positive. By using restriction endonucleases the K88-positive strains were in 4 cases classified as K88ab variant, 180 as K88ac variant and none contained gene for the K88ad variant. Ninety-five % coincidence with serological examination using K88ab, K88ac and K88ad specific antibodies was shown.

• MeSH
• Antigens, Bacterial analysis   classification   genetics   MeSH
• DNA Primers MeSH
• Escherichia coli classification   genetics   isolation & purification   MeSH
• Genetic Variation MeSH
• Escherichia coli Infections microbiology   veterinary   MeSH
• Swine Diseases microbiology   MeSH
• Polymerase Chain Reaction methods   MeSH
• Swine MeSH
• Fimbriae Proteins analysis   classification   genetics   MeSH
• Escherichia coli Proteins analysis   classification   genetics   MeSH
• Diarrhea microbiology   veterinary   MeSH
• DNA Restriction Enzymes metabolism   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia MeSH
• Names of Substances
• Antigens, Bacterial MeSH
• DNA Primers MeSH
• K88 antigen, E coli MeSH Browser
• Fimbriae Proteins MeSH
• Escherichia coli Proteins MeSH
• DNA Restriction Enzymes MeSH

The effect of O-ethyl-S-(2-diisopropylaminoethyl) methylthiophosphonate on rumen bacteria and rumen protozoa was investigated in sheep (after premedication with clinoptilolite-rich zeolite and without that premedication). In control animals a decrease in the total concentration of rumen protozoa was observed 3-7 d after intoxication (particularly in small and large ones). In clinoptilolite-rich-zeolite-treated animals only a slight decrease in protozoan numbers occurred during the first hours after the intoxication. Similarly, in every category of rumen bacteria marked differences between the groups were recorded, particularly in concentration of lipolytic bacteria. The results suggest some protective effect of clinoptilolite-rich zeolite for rumen microbiota against the organophosphate poison.

• MeSH
• Rumen drug effects   microbiology   parasitology   MeSH
• Bacteria drug effects   isolation & purification   MeSH
• Eukaryota drug effects   isolation & purification   MeSH
• Organothiophosphorus Compounds toxicity   MeSH
• Sheep MeSH
• Colony Count, Microbial MeSH
• Zeolites pharmacology   MeSH
• Animals MeSH
• Check Tag
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• clinoptilolite MeSH Browser
• Organothiophosphorus Compounds MeSH
• VX MeSH Browser
• Zeolites MeSH