Primers were designed and prepared and conditions were determined for PCR detection and differentiation of enterotoxigenic E. coli bacterial strains isolated from diarrheic pigs. Primers K88/1 and K88/2 are 25 bp oligomers that correspond to a region of genes encoding one of serological variants of the K88 antigen (K88ab(1), K88ab(2), K88ac or K88ad). A positive result of PCR is an amplificate of 792 bp in size for K88ab and K88ad variant or 786 bp for K88ac variant. The individual serological variants of genes of the K88 antigen could be differentiated by cutting the obtained PCR amplificates by restriction endonucleases. The PCR analysis of 674 E. coli strains isolated from diarrheic pigs showed that 184 strains were K88 positive. By using restriction endonucleases the K88-positive strains were in 4 cases classified as K88ab variant, 180 as K88ac variant and none contained gene for the K88ad variant. Ninety-five % coincidence with serological examination using K88ab, K88ac and K88ad specific antibodies was shown.

• MeSH
• antigeny bakteriální analýza   klasifikace   genetika   MeSH
• DNA primery MeSH
• Escherichia coli klasifikace   genetika   izolace a purifikace   MeSH
• genetická variace MeSH
• infekce vyvolané Escherichia coli mikrobiologie   veterinární   MeSH
• nemoci prasat mikrobiologie   MeSH
• polymerázová řetězová reakce metody   MeSH
• prasata MeSH
• proteiny fimbrií analýza   klasifikace   genetika   MeSH
• proteiny z Escherichia coli analýza   klasifikace   genetika   MeSH
• průjem mikrobiologie   veterinární   MeSH
• restrikční enzymy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH
• Názvy látek
• antigeny bakteriální MeSH
• DNA primery MeSH
• K88 antigen, E coli MeSH Prohlížeč
• proteiny fimbrií MeSH
• proteiny z Escherichia coli MeSH
• restrikční enzymy MeSH

The effect of O-ethyl-S-(2-diisopropylaminoethyl) methylthiophosphonate on rumen bacteria and rumen protozoa was investigated in sheep (after premedication with clinoptilolite-rich zeolite and without that premedication). In control animals a decrease in the total concentration of rumen protozoa was observed 3-7 d after intoxication (particularly in small and large ones). In clinoptilolite-rich-zeolite-treated animals only a slight decrease in protozoan numbers occurred during the first hours after the intoxication. Similarly, in every category of rumen bacteria marked differences between the groups were recorded, particularly in concentration of lipolytic bacteria. The results suggest some protective effect of clinoptilolite-rich zeolite for rumen microbiota against the organophosphate poison.

• MeSH
• bachor účinky léků   mikrobiologie   parazitologie   MeSH
• Bacteria účinky léků   izolace a purifikace   MeSH
• Eukaryota účinky léků   izolace a purifikace   MeSH
• organothiofosforové sloučeniny toxicita   MeSH
• ovce MeSH
• počet mikrobiálních kolonií MeSH
• zeolity farmakologie   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• clinoptilolite MeSH Prohlížeč
• organothiofosforové sloučeniny MeSH
• VX MeSH Prohlížeč
• zeolity MeSH