Bacterial species of the genus Prevotella represent a numerically dominant microbial population in the rumen of cattle. They belong to the phylogenetic division Cytophaga-Flexibacter-Bacteroides (CFB) which is a large group of ecologically diverse bacteria with only a few shared traits. The phylogenetic descent from a common ancestor seems to be unquestionable, however, as judged from the small subunit ribosomal RNA analysis. Only 4 ruminal Prevotella species have been described to date, even though the sequence analysis of directly retrieved 16S rRNA genes indicates a large genetic diversity within this group of rumen bacteria. The closest relatives of ruminal Prevotella spp. are not surprisingly other species of the genus Prevotella, typically inhabiting the gastrointestinal tract, oral cavity and genital areas of other animals and man. The previous phylogenetic analysis showed that species of the genus Prevotella can be split into two groups or superclusters, the "ruminal" and the "non-ruminal prevotellas". One of 4 currently described ruminal Prevotella spp., i.e. P. albensis, has been placed outside the supercluster containing ruminal Prevotella spp. and within the supercluster containing the non-ruminal Prevotella spp. However, the number of available small subunit rRNA sequences from this species represents only a fraction of all known ruminal Prevotella sequences.

• MeSH
• Rumen microbiology   MeSH
• DNA, Bacterial genetics   MeSH
• Phylogeny * MeSH
• Humans MeSH
• Evolution, Molecular * MeSH
• Prevotella classification   genetics   MeSH
• DNA, Ribosomal genetics   MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• Cattle MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Cattle MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• DNA, Bacterial MeSH
• DNA, Ribosomal MeSH
• RNA, Ribosomal, 16S MeSH

Data for bacterial identification were provided by culturing anaerobic bacteria under standardized conditions followed by extraction and methylation of cellular long-chain fatty acids and gas chromatographic analysis. The databases of fatty acid methyl ester (FAMEs) profiles for two predominant ruminal genera, Prevotella and Butyrivibrio, were created. Major long-chain cellular fatty acids found in the 23 analyzed Prevotella strains were 15:0 (anteiso), 15:0, 15:0 (iso) and 16:0. The strains of Prevotella could be well identified on species level by the characteristic ratios among major fatty acids and by acids unique fatty for each species. The 45 Butyrivibrio strains were grouped into 4 major and 2 minor groups according to FAMEs profiles. The major fatty acids for the bulk of the Butyrivibrio strains were 14:0, 15:1, 16:0 and 16:0 (iso). This groups corresponded to those based on 16S rDNA sequences.

• MeSH
• Rumen microbiology   MeSH
• Databases, Factual MeSH
• Gram-Negative Anaerobic Straight, Curved, and Helical Rods chemistry   classification   MeSH
• Fatty Acids analysis   MeSH
• Prevotella chemistry   classification   MeSH
• Bacterial Typing Techniques * MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Fatty Acids MeSH