Molecular monitoring of ruminal prevotellas
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
11501487
DOI
10.1007/bf02825895
Knihovny.cz E-zdroje
- MeSH
- bachor mikrobiologie MeSH
- fluorescenční barviva metabolismus MeSH
- fluorescenční mikroskopie MeSH
- hybridizace in situ metody MeSH
- oligonukleotidové sondy genetika MeSH
- polymerázová řetězová reakce metody MeSH
- Prevotella klasifikace genetika izolace a purifikace MeSH
- RNA ribozomální 16S genetika MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fluorescenční barviva MeSH
- oligonukleotidové sondy MeSH
- RNA ribozomální 16S MeSH
The development and preliminary use of two different molecular approaches for rapid enumeration and monitoring of ruminal prevotellas are described. Several oligonucleotide DNA probes, specific for the genus Prevotella and the species P. ruminicola and P. bryantii were labeled with various fluorochromes and used in in situ hybridization experiments. Epifluorescent microscopy was successfully used for the detection of fluorescent signal emitted by the probes in pure and mixed culture samples. The enumeration of the target cells and the analysis of the crude rumen fluid proved to be difficult, however, mainly due to the autofluorescent background and nonhomogeneous distribution of the cells on the microscope slide. The development of a competitive PCR system for ruminal prevotellas is described and the preliminary results of the rumen fluid analysis from a black-and-white Holstein cow are given.
Folia Microbiol (Praha) 2001;46(2):175 PubMed
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Systematics and evolution of ruminal species of the genus Prevotella
