Molecular monitoring of ruminal prevotellas
Language English Country United States Media print
Document type Journal Article
PubMed
11501487
DOI
10.1007/bf02825895
Knihovny.cz E-resources
- MeSH
- Rumen microbiology MeSH
- Fluorescent Dyes metabolism MeSH
- Microscopy, Fluorescence MeSH
- In Situ Hybridization methods MeSH
- Oligonucleotide Probes genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Prevotella classification genetics isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- Oligonucleotide Probes MeSH
- RNA, Ribosomal, 16S MeSH
The development and preliminary use of two different molecular approaches for rapid enumeration and monitoring of ruminal prevotellas are described. Several oligonucleotide DNA probes, specific for the genus Prevotella and the species P. ruminicola and P. bryantii were labeled with various fluorochromes and used in in situ hybridization experiments. Epifluorescent microscopy was successfully used for the detection of fluorescent signal emitted by the probes in pure and mixed culture samples. The enumeration of the target cells and the analysis of the crude rumen fluid proved to be difficult, however, mainly due to the autofluorescent background and nonhomogeneous distribution of the cells on the microscope slide. The development of a competitive PCR system for ruminal prevotellas is described and the preliminary results of the rumen fluid analysis from a black-and-white Holstein cow are given.
Folia Microbiol (Praha) 2001;46(2):175 PubMed
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