Castilleja tenuiflora is a native perennial plant used in traditional Mexican medicine. In June 2022, leaf blight symptoms were observed in a wild population of C. tenuiflora plants. Disease incidence was 80% and disease intensity reached up to 5% of the leaf area. Currently, there are no reports of pathogens causing leaf blight in this plant; therefore, this work aimed to identify the fungi responsible for the disease. The fungi recovered from the diseased tissue were characterized by means of pathogenicity tests and cultural, morphological, and molecular characterization. The information obtained revealed that Alternaria alternata and Alternaria gossypina are the pathogens responsible for the disease. This is the first report implicating species of Alternaria in causing leaf blight of C. tenuiflora in Mexico, as well as the first report of Alternaria gossypina also in Mexico. These pathogens may threaten the in situ conservation of native C. tenuiflora populations and limit their in vitro propagation. Future research lines should focus on determining the effect of these pathogens on metabolite production.

• Klíčová slova
• Alternaria, Mexican medicinal plants, Orobanchaceae, morphology, pathogenicity, phylogeny,
• Publikační typ
• časopisecké články MeSH

To evaluate the potential of using the enzymes from spent mushroom compost (SMC) as an industrial enzyme, the production of alpha-amylase, cellulase, beta-glucosidase, laccase, and xylanase was determined from the SMC of four edible mushroom species (Pleurotus ostreatus, Lentinula edodes, Flammulina velutipes and Hericium erinaceum). Among the tested SMC, the SMC of L. edodes showed the highest enzyme activity in alpha-amylase (229 nkat/g), cellulase (759 nkat/g) and beta-glucosidase (767 nkat/g) in 0.5% Triton X-100, and that of P. ostreatus showed the highest activity in laccase (1452 nkat/g) in phosphate-buffered 0.2% Triton X-100. The highest xylanase activity (119 nkat/g) was found in the SMC of F. velutipes.

• MeSH
• alfa-amylasy izolace a purifikace   metabolismus   MeSH
• Basidiomycota klasifikace   enzymologie   metabolismus   MeSH
• beta-glukosidasa izolace a purifikace   metabolismus   MeSH
• biodegradace MeSH
• celulasa izolace a purifikace   metabolismus   MeSH
• endo-1,4-beta-xylanasy izolace a purifikace   metabolismus   MeSH
• houby šii-take enzymologie   MeSH
• hydrolasy izolace a purifikace   metabolismus   MeSH
• lakasa izolace a purifikace   metabolismus   MeSH
• Pleurotus enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-amylasy MeSH
• beta-glukosidasa MeSH
• celulasa MeSH
• endo-1,4-beta-xylanasy MeSH
• hydrolasy MeSH
• lakasa MeSH