To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (2·0%) showed positive IgM reactions and seven samples (2·4%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (10·14%) for IgM and 36 samples (12·2%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0·006) and for IgG (P = 0·0007) antibodies. A statistically significant result was obtained for IgG (P = 0·02) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.

• Keywords
• Anaplasma phagocytophilum, Lyme borreliosis, human granulocytic anaplasmosis, major surface proteins, outer membrane protein A, tick-borne encephalitis,
• MeSH
• Anaplasma phagocytophilum immunology   MeSH
• Anaplasmosis diagnosis   immunology   microbiology   MeSH
• Bacterial Proteins immunology   MeSH
• Child MeSH
• Encephalitis, Tick-Borne immunology   microbiology   MeSH
• Coinfection diagnosis   immunology   microbiology   MeSH
• Humans MeSH
• Lyme Disease immunology   microbiology   MeSH
• Membrane Proteins immunology   MeSH
• Adolescent MeSH
• Child, Preschool MeSH
• Bacterial Outer Membrane Proteins immunology   MeSH
• Antibodies, Bacterial blood   MeSH
• Blotting, Western MeSH
• Check Tag
• Child MeSH
• Humans MeSH
• Adolescent MeSH
• Male MeSH
• Child, Preschool MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Czech Republic MeSH
• Names of Substances
• Bacterial Proteins MeSH
• Membrane Proteins MeSH
• Bacterial Outer Membrane Proteins MeSH
• Antibodies, Bacterial MeSH