In mature neurons, postsynaptic N-methyl-D-aspartate receptors (NMDARs) are segregated into two populations, synaptic and extrasynaptic, which differ in localization, function, and associated intracellular cascades. These two pools are connected via lateral diffusion, and receptor exchange between them modulates synaptic NMDAR content. Here, we identify the phosphorylation of the PDZ-ligand of the GluN2B subunit of NMDARs (at S1480) as a critical determinant in dynamically controlling NMDAR synaptic content. We find that phosphorylation of GluN2B at S1480 maintains NMDARs at extrasynaptic membranes as part of a protein complex containing protein phosphatase 1 (PP1). Global activation of NMDARs leads to the activation of PP1, which mediates dephosphorylation of GluN2B at S1480 to promote an increase in synaptic NMDAR content. Thus, PP1-mediated dephosphorylation of the GluN2B PDZ-ligand modulates the synaptic expression of NMDARs in mature neurons in an activity-dependent manner, a process with profound consequences for synaptic and structural plasticity, metaplasticity, and synaptic neurotransmission.

• Klíčová slova
• GluN2B, NMDA receptors, NMDAR synaptic content, PP1, dephosphorylation, extrasynaptic NMDAR,
• MeSH
• fosforylace MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• ligandy MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• neurony metabolismus   MeSH
• PDZ domény MeSH
• potkani Sprague-Dawley MeSH
• proteinfosfatasa 1 metabolismus   MeSH
• receptory N-methyl-D-aspartátu genetika   metabolismus   MeSH
• synapse metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• ligandy MeSH
• NR2B NMDA receptor MeSH Prohlížeč
• proteinfosfatasa 1 MeSH
• receptory N-methyl-D-aspartátu MeSH