Thirty-three isolates belonging to six species of the genus Trichoderma were tested for the ability to hydroxylate progesterone to 11alpha-, 11beta-, 11alpha,17alpha- and 6beta, 17alpha-derivatives, and epicortisol. T. aureoviride, T. harzianum, T. polysporum and T. pseudokoningii produced 11alpha-hydroxyprogesterone. T. harzianum and T. hamatum can form only the 11beta-isomer. T. koningii and T. hamatum produced 11alpha-, 11beta-, 11alpha,17alpha- and 6beta,11alpha-hydroxy derivatives. 11alpha, 11beta, 6beta,11alpha- and 11alpha,17alpha-hydroxyprogesterones and epicortisol are produced by T. aureoviride and T. pseudokoningii. Cortisol was produced only when the medium was fortified by 10 g/L peptone. This is the first record of conversion of progesterone to mono-, di- and trihydroxyprogesterones by these Trichoderma species.

• MeSH
• biotransformace MeSH
• hydroxylace MeSH
• progesteron chemie   metabolismus   MeSH
• Trichoderma růst a vývoj   izolace a purifikace   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• progesteron MeSH

The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates of Pleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.

• MeSH
• aktivace enzymů účinky léků   MeSH
• azosloučeniny metabolismus   MeSH
• lakasa metabolismus   MeSH
• látky znečišťující životní prostředí metabolismus   MeSH
• měď farmakologie   MeSH
• peroxidasy metabolismus   MeSH
• Pleurotus klasifikace   enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• azosloučeniny MeSH
• lakasa MeSH
• látky znečišťující životní prostředí MeSH
• manganese peroxidase MeSH Prohlížeč
• měď MeSH
• Orange G MeSH Prohlížeč
• peroxidasy MeSH