Nejvíce citovaný článek - PubMed ID 13411709
The chick-embryo model has been an important tool to study tumor growth, metastasis, and angiogenesis. However, an imageable model with a genetic fluorescent tag in the growing and spreading cancer cells that is stable over time has not been developed. We report here the development of such an imageable fluorescent chick-embryo metastatic cancer model with the use of green fluorescent protein (GFP). Lewis lung carcinoma cells, stably expressing GFP, were injected on the 12th day of incubation in the chick embryo. GFP-Lewis lung carcinoma metastases were visualized by fluorescence, after seven days additional incubation, in the brain, heart, and sternum of the developing chick embryo, with the most frequent site being the brain. The combination of streptokinase and gemcitabine was evaluated in this GFP metastatic model. Twelve-day-old chick embryos were injected intravenously with GFP-Lewis lung cancer cells, along with these two agents either alone or in combination. The streptokinase-gemcitabine combination inhibited metastases at all sites. The effective dose of gemcitabine was found to be 10 mg/kg and streptokinase 2000 IU per embryo. The data in this report suggest that this new stably fluorescent imageable metastatic-cancer chick-embryo model will enable rapid screening of new antimetastatic agents.
- MeSH
- deoxycytidin aplikace a dávkování analogy a deriváty MeSH
- gemcitabin MeSH
- karcinom plic Lewisové farmakoterapie MeSH
- kuřecí embryo MeSH
- léky antitumorózní - screeningové testy metody MeSH
- metastázy nádorů prevence a kontrola MeSH
- modely nemocí na zvířatech * MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- streptokinasa aplikace a dávkování MeSH
- zelené fluorescenční proteiny metabolismus MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- deoxycytidin MeSH
- gemcitabin MeSH
- streptokinasa MeSH
- zelené fluorescenční proteiny MeSH
