The aim of the present work was to construct a replication map of the chromosome of Mycobacterium phlei. The method of mutagenesis of the replication point by means of nitrosoguanidine was applied to synchronously multiplying populations. Back mutations and forward mutations were induced in auxotrophic mutants PA met and PA leu as well as in double auxotrophic mutants with methionine as the reference marker and the following order of replication of eleven genes on the chromosome was thus established: leu-Eth, Res-Stm, Cyk-pur-met, arg, Cyk-Bac-inl.

• MeSH
• antibiotická rezistence MeSH
• buněčné dělení MeSH
• DNA bakterií biosyntéza   MeSH
• geny MeSH
• leucin biosyntéza   MeSH
• mapování chromozomů * metody   MeSH
• methionin biosyntéza   MeSH
• methylnitronitrosoguanidin MeSH
• mutace MeSH
• mutageny MeSH
• Mycobacterium phlei * účinky léků   růst a vývoj   metabolismus   MeSH
• Mycobacterium * MeSH
• replikace DNA MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA bakterií MeSH
• leucin MeSH
• methionin MeSH
• methylnitronitrosoguanidin MeSH
• mutageny MeSH