We developed an alternative method of staining cell nuclei and chloroplast nucleoids of algal cells using SYBR Green I (the fluorescent dye used commonly for detecting dsDNA in agarose and polyacrylamide gels as an alternative to highly mutagenic ethidium bromide and for DNA staining of viruses and bacteria followed by flow cytometry, digital image analysis or real-time PCR), which enabled routine staining in vivo. Cells do not need to be fixed or treated chemically or physically before staining, thus the shape, size and position of DNA-containing structures are not affected. The fluorescence signal is sharp and reproducible. Examples of application of the method are shown in color microphotographs for representatives of eukaryotic algae from the taxa Chlorophyta, Rhodophyta and the prokaryotic Cyanophyta. The method is also useful for studying progress of the cell cycle in algal cells dividing by multiple fission, as shown by observation of changes in nuclear number during the cell cycle of the green alga Chlamydomonas reinhardtii and Scenedesmus quadricauda. Staining with SYBR Green I can be recommended as a fast, safe and efficient method for the detection of DNA-containing structures in vivo.

• MeSH
• barvení a značení metody   MeSH
• benzothiazoly MeSH
• buněčný cyklus MeSH
• chinoliny MeSH
• Chlorophyta genetika   fyziologie   ultrastruktura   MeSH
• diaminy MeSH
• DNA řas analýza   MeSH
• fluorescenční barviva metabolismus   MeSH
• organické látky metabolismus   MeSH
• permeabilita buněčné membrány MeSH
• počítačové zpracování obrazu MeSH
• polymerázová řetězová reakce MeSH
• průtoková cytometrie MeSH
• reprodukovatelnost výsledků MeSH
• Rhodophyta genetika   fyziologie   ultrastruktura   MeSH
• sinice genetika   fyziologie   ultrastruktura   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• benzothiazoly MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA řas MeSH
• fluorescenční barviva MeSH
• organické látky MeSH
• SYBR Green I MeSH Prohlížeč

Methods for determining the points of commitment for cell division are described for species of green algae dividing by multiple fission, both forming coenobia (Scenedesmus quadricauda) and releasing single daughter cells (Chlamydomonas eugametos, Scenedesmus armatus). The timing of commitment points was followed in detail in synchronous cultures of S. quadricauda grown under various light intensities, illumination regimes, and temperatures. The pre-commitment periods were rate limiting, while the post-commitment periods remained more or less constant under various light intensity. Temperature, on the other hand, affected both periods in a similar manner and they were prolonged with decreasing temperature.

• MeSH
• buněčné dělení * fyziologie   účinky záření   MeSH
• buněčný cyklus * MeSH
• Chlamydomonas cytologie   růst a vývoj   MeSH
• kultivační média MeSH
• Scenedesmus cytologie   růst a vývoj   MeSH
• světlo MeSH
• teplota MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• kultivační média MeSH