Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by β-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstrated that L-plastin participates in NKG2D-mediated inhibition of NK cell chemotaxis.

• Klíčová slova
• chemotaxis, membrane rafts,
• MeSH
• buněčná membrána účinky léků   metabolismus   MeSH
• buňky NK cytologie   metabolismus   MeSH
• centrifugace - gradient hustoty MeSH
• chemotaxe leukocytů fyziologie   MeSH
• detergenty farmakologie   MeSH
• kultivované buňky MeSH
• lektinové receptory NK-buněk - podrodina C metabolismus   MeSH
• lektinové receptory NK-buněk - podrodina K fyziologie   MeSH
• lidé MeSH
• malá interferující RNA farmakologie   MeSH
• membránové mikrodomény účinky léků   fyziologie   MeSH
• mikrofilamenta fyziologie   MeSH
• mikrofilamentové proteiny antagonisté a inhibitory   genetika   fyziologie   MeSH
• multiproteinové komplexy MeSH
• proteom MeSH
• receptory imunologické metabolismus   MeSH
• RNA interference MeSH
• signální transdukce imunologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• detergenty MeSH
• HCST protein, human MeSH Prohlížeč
• KLRK1 protein, human MeSH Prohlížeč
• LCP1 protein, human MeSH Prohlížeč
• lektinové receptory NK-buněk - podrodina C MeSH
• lektinové receptory NK-buněk - podrodina K MeSH
• malá interferující RNA MeSH
• mikrofilamentové proteiny MeSH
• multiproteinové komplexy MeSH
• proteom MeSH
• receptory imunologické MeSH