Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-phase wild-type Vpr-expressing S. pombe cells with a low, subinhibitory concentration (0.15 mmol/L) of hydrogen peroxide and 0.1 mmol/L thiamine significantly increased both cell proliferation and survival rates and decreased the number of elongated G2-arrested cells. Short-term, H2O2-induced adaptive stress increased the survival of the cells while acute stress conditions interrupted the Vpr-mediated death of the cells; however, no changes in cell length or cell phase were detected. The results suggest the importance of the oxidative status of the cells in Vpr-mediated processes. Our findings contribute to the development of a new approach via which to investigate the contribution of Vpr to HIV pathogenesis and to reduce the Vpr-mediated effects in HIV-infected patients.

• MeSH
• buněčný cyklus fyziologie   MeSH
• genové produkty vpr - virus lidské imunodeficience MeSH
• genové produkty vpr účinky léků   genetika   metabolismus   MeSH
• HIV-1 genetika   MeSH
• lidé MeSH
• oxidační stres fyziologie   MeSH
• peroxid vodíku farmakologie   MeSH
• proliferace buněk MeSH
• Schizosaccharomyces metabolismus   virologie   MeSH
• viabilita buněk MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• genové produkty vpr - virus lidské imunodeficience MeSH
• genové produkty vpr MeSH
• peroxid vodíku MeSH

Resistant strains of Escherichia coli were obtained by stepwise cultivation in media with increasing concentration of antimicrobially active 1-(methyldodecyl)dimethylamine oxide and 1-(methyldodecyl)trimethylammonium bromide. Adaptive changes were determined in the fatty-acid (FA) composition in an isolated lipopolysaccharide sample from the outer membrane of these strains. The composition of this FA mixture from adapted strains was compared with that of FA from a sensitive strain. The differences were found in level of palmitic, heptadecanoic, heptadecenoic, heptadecadienoic and nonadecenoic acids. In addition, the adapted strains differed from each other in the content of myristic, pentadecanoic, stearic and linoleic acids.

• MeSH
• bakteriální léková rezistence MeSH
• dimethylaminy farmakologie   MeSH
• Escherichia coli účinky léků   metabolismus   MeSH
• fyziologická adaptace účinky léků   MeSH
• kvartérní amoniové sloučeniny farmakologie   MeSH
• lipopolysacharidy metabolismus   MeSH
• mastné kyseliny metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dimethylaminy MeSH
• kvartérní amoniové sloučeniny MeSH
• lipopolysacharidy MeSH
• mastné kyseliny MeSH
• N-(1-methyldodecyl)-N,N,N-trimethylammonium MeSH Prohlížeč
• N,N-dimethyl-1-methyldodecylamine oxide MeSH Prohlížeč