Most cited article - PubMed ID 15942668
Use of polyclonal rabbit antibodies for detection of the bcr-abl fusion zone in cells transfected with experimental bcr-abl DNA vaccines
DNA vaccines showed great promise in preclinical models of infectious and malignant diseases, but their potency was insufficient in clinical trials and is needed to be improved. In this study, we tested systemic administration of two conventional adjuvants, synthetic oligodeoxynucleotide carrying immunostimulatory CpG motifs (CpG-ODN) and levamisole (LMS), and evaluated their effect on immune reactions induced by DNA vaccines delivered by a gene gun. DNA vaccination was directed either against the E7 oncoprotein of human papillomavirus type 16 or against the BCR-ABL1 oncoprotein characteristic for chronic myeloid leukemia. High doses of both adjuvants reduced activation of mouse splenic CD8(+) T lymphocytes, but the overall antitumor effect was enhanced in both tumor models. High-dose CpG-ODN exhibited a superior adjuvant effect in comparison with any combination of CpG-ODN with LMS. In summary, our results demonstrate the benefit of combined therapy with gene-gun-delivered antitumor DNA vaccines and systemic administration of CpG-ODN or LMS.
- MeSH
- Adjuvants, Immunologic administration & dosage MeSH
- Fusion Proteins, bcr-abl genetics immunology metabolism MeSH
- Biolistics MeSH
- CD8-Positive T-Lymphocytes drug effects metabolism pathology MeSH
- Vaccines, DNA MeSH
- Neoplasms, Experimental immunology pathology therapy MeSH
- Immunity drug effects MeSH
- Levamisole administration & dosage MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Mice, Inbred BALB C MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Oligodeoxyribonucleotides administration & dosage MeSH
- Papillomavirus E7 Proteins genetics immunology metabolism MeSH
- Cancer Vaccines * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Fusion Proteins, bcr-abl MeSH
- CPG-oligonucleotide MeSH Browser
- Vaccines, DNA MeSH
- Levamisole MeSH
- Oligodeoxyribonucleotides MeSH
- Papillomavirus E7 Proteins MeSH
- Cancer Vaccines * MeSH
