Relations between the diversity of the fliC gene conditioning flagellum protein in E. coli and the source of the strain origin are presented. The fliC genes have been identified and characterized in commensal E. coli derived from 10 healthy animal species living in Zoo Safari Park (Poland). The fliC gene was found in 150 strains by the PCR method. The amplifiedfliC products revealed single bands within the range 1.26-2.16 kbp. Forty restriction patterns (classed by restriction analysis with the use of RsaI (PCR-RFLP RsaI; R-types) were determined. The neighbor-joining method was employed to illustrate the distribution of the kinds of R-types. There are 3-8 various R-types of a diversified frequency of occurrence in strains. Application of PCR-RFLP RsaI permitted the identification of alleles of fliC genes characteristic for E. coli and the estimation of their diversity among the animal species. The transmission ways of E. coli fliC+ between organisms of different species were determined and confirmed the role of transmission and horizontal gene transfer in the generation of the allelic diversity of fliC gene in natural E. coli populations.

• MeSH
• alely MeSH
• Escherichia coli genetika   MeSH
• flagelin MeSH
• fylogeneze MeSH
• genetická variace genetika   MeSH
• infekce vyvolané Escherichia coli genetika   přenos   veterinární   MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• přenos genů horizontální MeSH
• proteiny z Escherichia coli genetika   MeSH
• savci mikrobiologie   MeSH
• zvířata v ZOO mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• flagelin MeSH
• FliC protein, E coli MeSH Prohlížeč
• proteiny z Escherichia coli MeSH