Aging is a complex process of organism decline in physiological functions. There is no clear theory explaining this phenomenon, but the most accepted one is the oxidative stress theory of aging. Biomarkers of oxidative stress, substances, which are formed during oxidative damage of phospholipids, proteins, and nucleic acids, are present in body fluids of diseased people as well as the healthy ones (in a physiological concentration). 8-iso prostaglandin F2α is the most prominent biomarker of phospholipid oxidative damage, o-tyrosine, 3-chlorotyrosine, and 3-nitrotyrosine are biomarkers of protein oxidative damage, and 8-hydroxy-2(')-deoxyguanosine and 8-hydroxyguanosine are biomarkers of oxidative damage of nucleic acids. It is thought that the concentration of biomarkers increases as the age of people increases. However, the concentration of biomarkers in body fluids is very low and, therefore, it is necessary to use a sensitive analytical method. A combination of HPLC and MS was chosen to determine biomarker concentration in three groups of healthy people of a different age (twenty, forty, and sixty years) in order to find a difference among the groups.

• MeSH
• biologické markery metabolismus   MeSH
• dinoprost analogy a deriváty   metabolismus   MeSH
• guanosin analogy a deriváty   metabolismus   MeSH
• lidé MeSH
• oxidační stres * MeSH
• poškození DNA MeSH
• proteiny chemie   metabolismus   MeSH
• stárnutí * MeSH
• tyrosin analogy a deriváty   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• 8-epi-prostaglandin F2alpha MeSH Prohlížeč
• biologické markery MeSH
• dinoprost MeSH
• guanosin MeSH
• proteiny MeSH
• tyrosin MeSH