In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality.

• Klíčová slova
• cumulus cells, gene expression, human, programmed cell death,
• MeSH
• analýza hlavních komponent MeSH
• biologické markery metabolismus   MeSH
• buněčná smrt genetika   MeSH
• buněčné kultury metody   MeSH
• časové faktory MeSH
• dospělí MeSH
• genová ontologie MeSH
• genové regulační sítě MeSH
• kumulární buňky cytologie   metabolismus   MeSH
• lidé MeSH
• regulace genové exprese MeSH
• reprodukovatelnost výsledků MeSH
• stanovení celkové genové exprese * MeSH
• stárnutí buněk genetika   MeSH
• tvar buňky genetika   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH