Characterization of classic type II restriction-modification systems (RMS) (restriction endonucleases and modification methyltransferases) was carried out in isolates of Staphylococcus aureus and Streptococcus agalactiae obtained from clinical material. Among the 100 isolates of S. aureus two different RMS type II were detected. The first was expressed in isolates 32 and 33 (Sau32 I and Sau33 I); the targeting sequence was determined as 5'-GGN CC-3' (Sau96 I isoschizomer). The second was found in isolates no. 90, 93, 96*, and 98 (Sau90 I, Sau93 I, Sau96* I, Sau98 I) and enzymes recognized sequence 5'-CTY RAG-3' (SmlI isoschizomer). Analysis of 40 isolates of S. agalactiae revealed only one RMS; it was detected in two isolates (no. 16 and 23; Sag16 I and Sag23 I). Restriction endonuclease expressed by these isolates cleaved DNA in sequence 5'-CTG CA/G-3' (PstI isoschizomer). In RMS-positive S. aureus and S. agalactiae isolates plasmid DNA capable of replication in Escherichia coli and Bacillus subtilis was also detected and isolated.

• MeSH
• DNA bakterií metabolismus   MeSH
• DNA restrikčně-modifikační enzymy genetika   metabolismus   MeSH
• hospodářská zvířata MeSH
• restrikční endonukleasy typu II genetika   metabolismus   MeSH
• stafylokokové infekce mikrobiologie   veterinární   MeSH
• Staphylococcus aureus enzymologie   izolace a purifikace   MeSH
• Streptococcus agalactiae enzymologie   izolace a purifikace   MeSH
• streptokokové infekce mikrobiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA bakterií MeSH
• DNA restrikčně-modifikační enzymy MeSH
• restrikční endonukleasy typu II MeSH