Characterization of classic type II restriction-modification systems (RMS) (restriction endonucleases and modification methyltransferases) was carried out in isolates of Staphylococcus aureus and Streptococcus agalactiae obtained from clinical material. Among the 100 isolates of S. aureus two different RMS type II were detected. The first was expressed in isolates 32 and 33 (Sau32 I and Sau33 I); the targeting sequence was determined as 5'-GGN CC-3' (Sau96 I isoschizomer). The second was found in isolates no. 90, 93, 96*, and 98 (Sau90 I, Sau93 I, Sau96* I, Sau98 I) and enzymes recognized sequence 5'-CTY RAG-3' (SmlI isoschizomer). Analysis of 40 isolates of S. agalactiae revealed only one RMS; it was detected in two isolates (no. 16 and 23; Sag16 I and Sag23 I). Restriction endonuclease expressed by these isolates cleaved DNA in sequence 5'-CTG CA/G-3' (PstI isoschizomer). In RMS-positive S. aureus and S. agalactiae isolates plasmid DNA capable of replication in Escherichia coli and Bacillus subtilis was also detected and isolated.

Institute of Molecular Biology Center of Excellence for Molecular Medicine Slovak Academy of Sciences 845 51 Bratislava Slovakia

Zobrazit více v PubMed

Mol Gen Genet. 1979 Jan 5;168(1):111-5 PubMed

Annu Rev Microbiol. 1965;19:365-78 PubMed

Microbiology (Reading). 1999 Jan;145 ( Pt 1):127-134 PubMed

Folia Microbiol (Praha). 2003;48(3):319-28 PubMed

FEMS Microbiol Lett. 1993 Oct 15;113(2):129-32 PubMed

FEMS Microbiol Lett. 1998 Jul 1;164(1):215-8 PubMed

Mol Gen Genet. 1996 Nov 27;253(1-2):74-80 PubMed

Folia Microbiol (Praha). 2001;46(3):193-6 PubMed

J Bacteriol. 1988 Jun;170(6):2533-6 PubMed

Curr Microbiol. 2001 Feb;42(2):122-8 PubMed

Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PubMed

Zentralbl Bakteriol. 1998 May;287(4):277-314 PubMed

J Mol Biol. 1973 Dec 15;81(3):419-23 PubMed

Nucleic Acids Res. 2001 Jan 1;29(1):268-9 PubMed

Anaerobe. 1999 Feb;5(1):37-41 PubMed

Arch Microbiol. 1994;161(5):439-41 PubMed

J Basic Microbiol. 1991;31(2):141-7 PubMed

Nucleic Acids Res. 1992 Aug 25;20(16):4364 PubMed

FEMS Microbiol Lett. 1996 May 1;138(2-3):123-7 PubMed

Nucleic Acids Res. 1993 Jul 1;21(13):3139-54 PubMed

Nucleic Acids Res. 1993 Oct 11;21(20):4843 PubMed

Folia Microbiol (Praha). 2001;46(6):483-7 PubMed

Microbiol Rev. 1993 Jun;57(2):434-50 PubMed

Folia Microbiol (Praha). 2002;47(6):641-8 PubMed

Lett Appl Microbiol. 1993 Dec;17(6):297-9 PubMed

Toll-like receptor gene polymorphism and its relationship with somatic cell concentration and natural bacterial infections of the mammary gland in sheep

Successful eradication of methicillin-resistant Staphylococcus aureus (MRSA) intestinal carrier status in a healthcare worker--case report

The use of bacteriophages in eliminating polyresistant strains of Staphylococcus aureus and Streptococcus agalactiae

Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity