The use of bacteriophages in eliminating polyresistant strains of Staphylococcus aureus and Streptococcus agalactiae
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16295655
DOI
10.1007/bf02931564
Knihovny.cz E-zdroje
- MeSH
- aktivace viru MeSH
- DNA virů genetika izolace a purifikace MeSH
- lidé MeSH
- mnohočetná bakteriální léková rezistence genetika MeSH
- R-plasmidy genetika MeSH
- stafylokokové bakteriofágy fyziologie MeSH
- Staphylococcus aureus účinky léků genetika izolace a purifikace virologie MeSH
- Staphylococcus účinky léků genetika izolace a purifikace virologie MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA virů MeSH
Temperate bacteriophages were induced in and released from isolates of Staphylococcus aureus and Streptococcus agalactiae using mitomycin C. Various specific indicator cultures were tested for providing clear plaques after phage infection. Specific lytic mixture of bacteriophages was prepared using the induced, modified and laboratory variants of phages. Under laboratory conditions, the mixture eliminated all isolates from the tested collection of microorganisms. The restriction barrier of some bacterial isolates to bacteriophage infection was overcome either by UV irradiation or in vitro modification of bacteriophage DNA with specific methyltransferases. Conjugative R plasmids, capable of replication in G+ and G- bacteria, were detected and isolated from S. aureus and S. agalactiae antibiotic-resistant strains.
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