A rapid screening method for detecting active compounds against erythromycin-resistant bacterial strains of Finnish origin
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
16681145
DOI
10.1007/bf02931435
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky chemie farmakologie MeSH
- bakteriální léková rezistence * MeSH
- časové faktory MeSH
- erythromycin farmakologie MeSH
- flavonoidy farmakologie MeSH
- hydroxybenzoáty farmakologie MeSH
- kumariny farmakologie MeSH
- kyselina gallová analogy a deriváty farmakologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti metody normy MeSH
- nefelometrie a turbidimetrie MeSH
- penicilin G farmakologie MeSH
- Staphylococcus účinky léků růst a vývoj MeSH
- Streptococcus pyogenes účinky léků růst a vývoj MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Finsko MeSH
- Názvy látek
- antibakteriální látky MeSH
- erythromycin MeSH
- flavonoidy MeSH
- hydroxybenzoáty MeSH
- kumariny MeSH
- kyselina gallová MeSH
- lauryl gallate MeSH Prohlížeč
- octyl gallate MeSH Prohlížeč
- penicilin G MeSH
- phenolic acid MeSH Prohlížeč
A rapid and simple microdilution technique on 96-well microplate based on turbidimetry was optimized and validated for screening of antimicrobial activity against erythromycin-resistant bacterial strains of Streptococcus pyogenes and Staphylococcus simulans isolated from Finnish patients. Using S. pyogenes ATCC 12351 as reference strain the developed method was evaluated by reproducibility measurements and using parameters typically employed for screening methods, i.e. signal-to-background, signal-to-noise and a screening-window coefficient, the Z' factor. The method was further used for screening a group of natural compounds and their synthetic derivatives against resistant bacterial strains. Of these, octyl and dodecyl gallates, and usnic and ursolic acids were the most active. The described method is a rapid, homogeneous, cost-effective and easy-to-perform system for screening of new potential antimicrobial agents in drug discovery.
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