A rapid screening method for detecting active compounds against erythromycin-resistant bacterial strains of Finnish origin
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16681145
DOI
10.1007/bf02931435
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- Drug Resistance, Bacterial * MeSH
- Time Factors MeSH
- Erythromycin pharmacology MeSH
- Flavonoids pharmacology MeSH
- Hydroxybenzoates pharmacology MeSH
- Coumarins pharmacology MeSH
- Gallic Acid analogs & derivatives pharmacology MeSH
- Humans MeSH
- Microbial Sensitivity Tests methods standards MeSH
- Nephelometry and Turbidimetry MeSH
- Penicillin G pharmacology MeSH
- Staphylococcus drug effects growth & development MeSH
- Streptococcus pyogenes drug effects growth & development MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Finland MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Erythromycin MeSH
- Flavonoids MeSH
- Hydroxybenzoates MeSH
- Coumarins MeSH
- Gallic Acid MeSH
- lauryl gallate MeSH Browser
- octyl gallate MeSH Browser
- Penicillin G MeSH
- phenolic acid MeSH Browser
A rapid and simple microdilution technique on 96-well microplate based on turbidimetry was optimized and validated for screening of antimicrobial activity against erythromycin-resistant bacterial strains of Streptococcus pyogenes and Staphylococcus simulans isolated from Finnish patients. Using S. pyogenes ATCC 12351 as reference strain the developed method was evaluated by reproducibility measurements and using parameters typically employed for screening methods, i.e. signal-to-background, signal-to-noise and a screening-window coefficient, the Z' factor. The method was further used for screening a group of natural compounds and their synthetic derivatives against resistant bacterial strains. Of these, octyl and dodecyl gallates, and usnic and ursolic acids were the most active. The described method is a rapid, homogeneous, cost-effective and easy-to-perform system for screening of new potential antimicrobial agents in drug discovery.
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