Genetic heterogeneity of the pathogenic potentials of human and bovine group B streptococci
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
AI19304
NIAID NIH HHS - United States
TW00188
FIC NIH HHS - United States
PubMed
12094741
DOI
10.1007/bf02817655
Knihovny.cz E-zdroje
- MeSH
- bakteriální geny MeSH
- genetická variace MeSH
- lidé MeSH
- polymerázová řetězová reakce MeSH
- pulzní gelová elektroforéza MeSH
- senzitivita a specificita MeSH
- skot MeSH
- Streptococcus agalactiae klasifikace genetika patogenita MeSH
- streptokokové infekce epidemiologie mikrobiologie MeSH
- techniky typizace bakterií ekonomika metody MeSH
- virulence genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
One-hundred seventy-two B-streptococcal strains of human and bovine origin were analyzed for the presence of 9 genes potentially involved in virulence. Some of genes (glnA, cyl, hylB, scaA and cfb) were revealed in all the strains. However, the presence of others (bca, bac, scpB, lmb) varied from strain to strain. Taken together, 3 and 5 different types of pathogenic potential were found among human and bovine group B streptococci (GBS) strains, respectively, and only one type (bca+ bac scpB+ glnA+ cyl+ hylB+ lmb+ scaA+ cfb+) was common for both kinds of strains. We propose that different virulence genes can be involved in the development of infectious processes in humans and animals. A reliable PCR protocol with 3 pairs of primers (for the genes bca, bac and scpB) in the same reaction mixture was developed for the fast identification of the pathogenic potential of GBS. In comparison with the classical immunological methods this procedure displayed higher specificity and sensitivity as well as a shorter time of analysis. It can be recommended for use in the clinical and veterinary practice for studying the epidemiological relationship between the isolates and the ready identification of the clone causing the infection.
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Can Vet J. 1997 Jul;38(7):429-37 PubMed
Rev Infect Dis. 1988 Jul-Aug;10 Suppl 2:S363-6 PubMed
J Clin Microbiol. 2000 Jan;38(1):71-8 PubMed
J Clin Microbiol. 1993 Mar;31(3):762-4 PubMed
J Clin Microbiol. 1998 Jul;36(7):2115-6 PubMed
J Hyg (Lond). 1966 Jun;64(2):191-203 PubMed
Protein Sci. 1996 Sep;5(9):1939-41 PubMed
Folia Microbiol (Praha). 1999;44(4):449-53 PubMed
Adv Exp Med Biol. 1997;418:343-6 PubMed
FEMS Immunol Med Microbiol. 1997 Mar;17(3):149-54 PubMed
Folia Microbiol (Praha). 1999;44(6):726-8 PubMed
Annu Rev Microbiol. 2000;54:641-79 PubMed
Mol Microbiol. 2000 Sep;37(6):1444-55 PubMed
Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity
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