Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human-Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes.

• Klíčová slova
• Arabidopsis genome, DNA methylation, epigenome, gene expression, human–plant hybrid cell line, whole-genome bisulfite sequencing (WGBS),
• MeSH
• Arabidopsis genetika   MeSH
• buněčné linie MeSH
• chromozomy rostlin genetika   MeSH
• DNA rostlinná genetika   MeSH
• epigeneze genetická genetika   MeSH
• epigenom genetika   MeSH
• epigenomika metody   MeSH
• genom rostlinný genetika   MeSH
• hybridní buňky fyziologie   MeSH
• lidé MeSH
• methyltransferasy genetika   MeSH
• metylace DNA genetika   MeSH
• repetitivní sekvence nukleových kyselin genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA rostlinná MeSH
• methyltransferasy MeSH