To elucidate the physiological meaning of OmpR-dependent expression of invasin gene (inv) inhibition in Yersinia enterocolitica, the function of the EnvZ/OmpR regulatory pathway in osmoregulation of inv expression was analyzed in detail. The osmoregulation of inv expression was found to be a multifaceted process involving both OmpR-dependent and -independent mechanisms. Analysis of inv transcription in strains lacking OmpR or EnvZ proteins indicated that kinase EnvZ is not the only regulator of OmpR phosphorylation. Using the transcriptional inv::lacZ fusion in a heterologous system (Escherichia coli) we tried to clarify the role of OmpR in the inv regulatory circuit composed of negative (H-NS) and positive (RovA) regulators of inv gene transcription. We were able to show a significant increase in inv expression in E. coli ompR background under H-NS( Ecoli )-repressed condition. Moreover, H-NS-mediated inv repression was relieved when RovA of Y. enterocolitica was expressed from a plasmid. Furthermore, we showed that RovA may activate inv expression irrespective on the presence of H-NS protein. Using this strategy we showed that OmpR of Y. enterocolitica decrease RovA-mediated inv activation.

• MeSH
• bakteriální adheziny genetika   MeSH
• bakteriální chromozomy genetika   MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• Escherichia coli genetika   metabolismus   MeSH
• fúze genů MeSH
• genetická transkripce MeSH
• lac operon genetika   MeSH
• osmolární koncentrace MeSH
• promotorové oblasti (genetika) MeSH
• regulace genové exprese u bakterií * MeSH
• trans-aktivátory genetika   metabolismus   MeSH
• Yersinia enterocolitica genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální adheziny MeSH
• bakteriální proteiny MeSH
• invasin, Yersinia MeSH Prohlížeč
• osmolarity response regulator proteins MeSH Prohlížeč
• trans-aktivátory MeSH