The emergence of West Nile virus (WNV) and Usutu virus (USUV) in addition to the autochthonous tick-borne encephalitis virus (TBEV) in Europe causes rising concern for public and animal health. The first equine case of West Nile neuroinvasive disease in Austria was diagnosed in 2016. As a consequence, a cross-sectional seroprevalence study was conducted in 2017, including 348 equids from eastern Austria. Serum samples reactive by ELISA for either flavivirus immunoglobulin G or M were further analyzed with the plaque reduction neutralization test (PRNT-80) to identify the specific etiologic agent. Neutralizing antibody prevalences excluding vaccinated equids were found to be 5.3% for WNV, 15.5% for TBEV, 0% for USUV, and 1.2% for WNV from autochthonous origin. Additionally, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to detect WNV nucleic acid in horse sera and was found to be negative in all cases. Risk factor analysis did not identify any factors significantly associated with seropositivity.

• Klíčová slova
• Austria, Usutu virus, West Nile virus, epidemiology, flavivirus, horses, seroprevalence, tick-borne encephalitis virus,
• MeSH
• endemické nemoci veterinární   MeSH
• Equidae virologie   MeSH
• Flavivirus imunologie   MeSH
• infekce viry z rodu Flavivirus epidemiologie   veterinární   MeSH
• klíšťová encefalitida epidemiologie   veterinární   MeSH
• koně MeSH
• nemoci koní epidemiologie   MeSH
• protilátky virové krev   MeSH
• průřezové studie MeSH
• rizikové faktory MeSH
• séroepidemiologické studie MeSH
• virus západního Nilu imunologie   MeSH
• viry klíšťové encefalitidy imunologie   MeSH
• západonilská horečka epidemiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Rakousko epidemiologie   MeSH
• Názvy látek
• protilátky virové MeSH