High levels of an aromatic nitrilase (about 37 microkat/L culture) were induced in Fusarium solani O1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/L picolinonitrile. The mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (LentiKats). The cell-free extract was immobilized by hydrophobic binding onto a Butyl Sepharose column. The enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonitrile and benzonitrile.

• MeSH
• aminohydrolasy biosyntéza   MeSH
• Fusarium enzymologie   růst a vývoj   MeSH
• hydrolýza MeSH
• imobilizované buňky metabolismus   mikrobiologie   MeSH
• nitrily metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aminohydrolasy MeSH
• nitrilase MeSH Prohlížeč
• nitrily MeSH