Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.

• MeSH
• disacharidy izolace a purifikace   MeSH
• extracelulární prostor chemie   metabolismus   MeSH
• glykosylace MeSH
• kultivační média chemie   MeSH
• kyseliny glycerové agonisté   chemie   izolace a purifikace   metabolismus   MeSH
• magnetická rezonanční spektroskopie MeSH
• osmolární koncentrace MeSH
• osmóza MeSH
• Streptomyces chemie   metabolismus   MeSH
• trehalosa chemie   izolace a purifikace   metabolismus   MeSH
• vodní a elektrolytová rovnováha * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• disacharidy MeSH
• glyceric acid MeSH Prohlížeč
• kultivační média MeSH
• kyseliny glycerové MeSH
• trehalosa MeSH