To elucidate the physiological meaning of OmpR-dependent expression of invasin gene (inv) inhibition in Yersinia enterocolitica, the function of the EnvZ/OmpR regulatory pathway in osmoregulation of inv expression was analyzed in detail. The osmoregulation of inv expression was found to be a multifaceted process involving both OmpR-dependent and -independent mechanisms. Analysis of inv transcription in strains lacking OmpR or EnvZ proteins indicated that kinase EnvZ is not the only regulator of OmpR phosphorylation. Using the transcriptional inv::lacZ fusion in a heterologous system (Escherichia coli) we tried to clarify the role of OmpR in the inv regulatory circuit composed of negative (H-NS) and positive (RovA) regulators of inv gene transcription. We were able to show a significant increase in inv expression in E. coli ompR background under H-NS( Ecoli )-repressed condition. Moreover, H-NS-mediated inv repression was relieved when RovA of Y. enterocolitica was expressed from a plasmid. Furthermore, we showed that RovA may activate inv expression irrespective on the presence of H-NS protein. Using this strategy we showed that OmpR of Y. enterocolitica decrease RovA-mediated inv activation.

• MeSH
• Adhesins, Bacterial genetics   MeSH
• Chromosomes, Bacterial genetics   MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• Escherichia coli genetics   metabolism   MeSH
• Gene Fusion MeSH
• Transcription, Genetic MeSH
• Lac Operon genetics   MeSH
• Osmolar Concentration MeSH
• Promoter Regions, Genetic MeSH
• Gene Expression Regulation, Bacterial * MeSH
• Trans-Activators genetics   metabolism   MeSH
• Yersinia enterocolitica genetics   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Adhesins, Bacterial MeSH
• Bacterial Proteins MeSH
• invasin, Yersinia MeSH Browser
• osmolarity response regulator proteins MeSH Browser
• Trans-Activators MeSH