Biodegradable hydrogels are studied as potential scaffolds for soft tissue regeneration. In this work biodegradable hydrogels were prepared from synthetic poly(α-amino acid)s, poly(AA)s. The covalently crosslinked gels were formed by radical copolymerization of methacryloylated poly(AA)s, e.g. poly[N (5)-(2-hydroxy-ethyl)-L-glutamine-ran-L-alanine-ran-N (6)-methacryloyl-L-lysine], as a multifunctional macro-monomer with a low-molecular-weight methacrylic monofunctional monomer, e.g. 2-hydroxyethyl methacrylate (HEMA). Methacryloylated copolypeptides were synthesized by polymerization of N-carboxyanhydrides of respective amino acids and subsequent side-chain modification. Due to their polypeptide backbone, synthetic poly(AA)s are cleavable in biological environment by enzyme-catalyzed hydrolysis. The feasibility of enzymatic degradation of poly(AA)s alone and the hydrogels made from them was studied using elastase, a matrix proteinase involved in tissue healing processes, as a model enzyme. Specificity of elastase for cleavage of polypeptide chains behind the L-alanine residues was reflected in faster degradation of L-alanine-containing copolymers as well as of hydrogels composed of them.

• MeSH
• aminokyseliny chemie   MeSH
• biodegradace MeSH
• biokompatibilní materiály chemie   MeSH
• časové faktory MeSH
• chemické modely MeSH
• chrupavka patologie   MeSH
• gely MeSH
• hydrogely chemie   MeSH
• magnetická rezonanční spektroskopie MeSH
• methakryláty chemie   MeSH
• pankreatická elastasa chemie   MeSH
• peptidy chemie   MeSH
• polymery chemie   MeSH
• reagencia zkříženě vázaná chemie   MeSH
• regenerace nervu MeSH
• tkáňové inženýrství přístrojové vybavení   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aminokyseliny MeSH
• biokompatibilní materiály MeSH
• gely MeSH
• hydrogely MeSH
• hydroxyethyl methacrylate MeSH Prohlížeč
• methakryláty MeSH
• pankreatická elastasa MeSH
• peptidy MeSH
• polymery MeSH
• reagencia zkříženě vázaná MeSH